A comparative Pharmacognostical Study of certain Jasminum Species
Jasminum is a genus of flowering climbing shrubs and vines (family Oleaceae). Genus Jasminum composed of over 2000 plants distributed all over the world. They are native to Eurasia, India, and the Mediterranian region. Jasminum. azoricum L., Jasminum humile L., Jasminum multiflorum Burm.f., Jasminum officinale L., Jasminum sambac L. (Arabian nights) and Jasminum sambac L. (Grand Duke of Tuscany) were collected from AL-Keram farms, Al-Beheira Government.The plants; were subjected to DNA fingerprinting using SCOT and ISSR primers for genetic characterization. The study was performed on biologically guided bases, so four in-vitro biological studies were performed; antioxidant, antimicrobial, cytotoxic effect on three cell lines and antihepatic C virus. The comparative biological studies showed that J. multiflorum leaves have higher antioxidant effects by DPPH assay, while J. officinale and J. sambac (G) flower extracts showed more antimicrobial effects than other species and cultivars. The cytotoxicity study showed that all the species possessed cytotoxic effects in different degrees, the higher cytotoxic effects observed in J. humile and J. sambac. In-vitro HCV activity was performed in addition to cell genes analysis by western blot technique, proved that J. multiflorum leaves and flowers can decrease the viral load and inhibit its replication by 85-88%. They inhibit viral replication by affecting NS5A protein and inhibit viral penetration and expansion of infection by affecting the EPAH2 gene.Comparative phytochemical investigations were performed on the six Jasminum plants, starting with phytochemical screening for the flowers and leaves. The HPLC-PDA-ESI-MS/MS technique was used to explore the chemical composition of the leaves, a total of 115 compounds were determined tentatively with interspecies variations, different classes of compounds were identified; 5 phenylethanoids, 19 simple phenols, 18 flavonoids, 7 lignans, and 66 secoiridoids. Estimation of flavonoids and phenolic component in each plant showed the highest phenolic contents in J. multiflorum, while similarity in flavonoid contents was observed in the six plants. Volatile components of the six plants were investigated using solid phase microextraction technique, that showed the interspecies differences of volatile oil composition among selected species and cultivars, the major components were; E,E-α-Farnesene, benzoyl acetate and cis-3-hexenyl benzoate. The quantitative determination of some phenolic acids and flavonoids of the methanolic extracts of leaves were performed using HPLC-DAD method.