Suchergebnisse

We acknowledge the financial support from the Spanish Ministerio de Ciencia y Universidades (RTI-2018-095756-BI00), Principado de Asturias Government (IDI2018-000217), co-financed by FEDER funds, ERA-NET COFUND Photonicsensing, Safe-water GA n. 688735 and Fondazione CR Firenze ID 2018.0944.

We are grateful to the Spanish Ministerio de Economia y Competitividad (Project No. CTQ2015-63567-R), and the Principado de Asturias government (Project FC15-GRUPIN14-025, co-financed by FEDER funds) for their financial support of our research.

Spanish Government [RTI-2018-095756-B-I00]; Principado de Asturias Government [IDI2018-000217]; FEDER funds, European Commission; Spanish Ministerio de Ciencia, Innovacion y Universidades [MCIU-20-PRE2019-087618]

This research was funded by the Spanish Ministerio de Ciencia y Universidades, grant number RTI-2018-095756-B-I00, and Principado de Asturias Government, grant number IDI/2018/000217, co-financed by FEDER funds.

The authors acknowledge the support provided by the Spanish Ministerio de Economia y Competitividad (Project No. CTQ2015-63567-R), and the Principado de Asturias government (Project FC15-GRUPIN14-025, co-financed by FEDER funds).

This work was financially supported by the Spanish Ministerio de Economía y Competitividad (Project No. CTQ2015-63567-R), the Principado de Asturias government (Project FC15-GRUPIN14- 025), and co-financed by FEDER funds.

The Principado de Asturias government, under the project FC15-GRUPIN14-025 co-financed by FEDER funds, provided financial support. RMC also thanks the Principado de Asturias government and FICYT for a Clarín post-doctoral contract.

This research was funded by the Spanish Government (project RTI-2018-095756-B-I00) and Principado de Asturias Government (IDI2018-000217), co-financed by FEDER funds. R.L.-G. thanks the Spanish Government for a predoctoral FPU fellowship (FPU16/05670).

This research was supported by the Spanish Ministerio de Economía y Competitividad through the Project CTQ2015-63567-R, Principado de Asturias government (Project FC15-GRUPIN-14-025) and co-financed by FEDER funds. Finanial support from the Italian Ministry of University and Scientific research is also gratefully acknowledged.

This work received financial support from the European Union (FEDER funds through COMPETE), National Funds (FCT, Fundação para a Ciência e a Tecnologia) through project UID/QUI/50006/2013 and Regional Funds (Principado de Asturias government through Project FC15-GRUPIN14-025), and cofinanced by FEDER funds. A.P. and M.F.B. are grateful to FCT grants SFRH/BD/97995/2013 and SFRH/BPD/78845/2011, financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. C.P. thanks FCT for the FCT Investigator contract IF/01080/2015.

This work was supported by Principado de Asturias government and FICYT under a CLARIN post-doctoral contract (RMC) and project FC15-GRUPIN14-025

Financial support from Principado de Asturias Government (IDI2018-000217), co-financed by FEDER funds, is acknowledged. We thank the support from Asociación de Celiacos y sensibles al gluten - Comunidad de Madrid.

The integration of nanomaterials in the field of (bio)sensors has allowed developing strategies with improved analytical performance. In this work, ultrasmall core-shell Fe3O4@Au magnetic nanoparticles (MNPs) were used as the platform for the immobilization of event-specific Roundup Ready (RR) soybean and taxon-specific DNA sequences. Firstly, monodisperse Fe3O4 MNPs were synthesized by thermal decomposition and subsequently coated with a gold shell through reduction of Au(III) precursor on the surface of the MNPs in the presence of an organic capping agent. This nanosupport exhibited high colloidal stability, average particle size of 10.2 ± 1.3 nm, and spherical shape. The covalent immobilization of ssDNA probe onto the Au shell of the Fe3O4@Au MNPs was achieved through a self-assembled monolayer (SAM) created from mixtures of alkane thiols (6-mercapto-1-hexanol and mercaptohexanoic acid). The influence of the thiols ratio on the electrochemical performance of the resulting electrochemical genoassays was studied, and remarkably, the best analytical performance was achieved for a pure mercaptohexanoic acid SAM. Two quantification assays were designed; one targeting an RR sequence and a second targeting a reference soybean gene, both with a sandwich format for hybridization, signaling probes labelled with fluorescein isothiocyanate (FITC), enzymatic amplification and chronoamperometric detection at screen-printed carbon electrodes (SPCE). The magnetogenoassays exhibited linear ranges from 0.1 to 10.0 nM and from 0.1 to 5.0 nM with similar detection limits of 0.02 nM and 0.05 nM for the event-specific (RR) and the taxon-specific (lectin) targets, respectively. The usefulness of the approach was demonstrated by its application to detect genetically modified organisms (GMOs) in feed and food. ; This work received financial support from the European Union (FEDER funds through COMPETE), National Funds (FCT, Fundação para a Ciência e a Tecnologia) through project UID/QUI/50006/2013 and Regional Funds (Principado de Asturias government through Project FC15-GRUPIN14-025), and cofinanced by FEDER funds. A.P. and M.F.B. are grateful to FCT grants SFRH/BD/97995/2013 and SFRH/BPD/78845/2011, financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. C.P. thanks FCT for the FCT Investigator contract IF/01080/2015. ; info:eu-repo/semantics/publishedVersion

This research was funded by the Spanish Government (project RTI-2018-095756-B-I00). RLG. thanks the Spanish Government for a PhD fellowship (FPU16/05670). We also thank Dr. M. García Ocana~ for his help and guidance in the experiments with cells, as well as the Sequencing Unit from the Scientific and Technological Resources of the University of Oviedo, where PCR, electrophoresis, cloning and sequencing experiments were performed, and the Proteomic Unit from the Health Research Institute of Santiago de Compostela (IDIS), where mass spectrometry analyses were carried out.

We thank the Biomedical and Biotechnological core facilities of University of Oviedo. We also thank Prof. R. de Llorens (UDG) for his kind donation of PhoSL lectin. We acknowledge the financial support by the Spanish Ministerio de Economía y Competitividad (CTQ2015-63567-R) and Ministerio de Ciencia y Universidades (RTI2018-095756-B-I00), as well as Principado de Asturias Government (IDI2018-000217 and IDI2018-000177), co-financed by FEDER funds. A. D. F. was supported by Asociación Española contra el Cáncer (AECC) with a PhD fellowship.