Suchergebnisse

Financial supports from the Spanish Ministry of Economy and Competitiveness (MEC, Project CTQ2016-75752-R), the Spanish Ministry of Science and Innovation (MCI, PID2019-109253RB-I00), and the Asturian Regional Government (FC-GRUPIN-IDI/2018/000181) are gratefully acknowledged.

Financial support from the Spanish Ministry of Economy and Competitiveness (MEC, Project CTQ2016-75752-R), the Spanish Ministry of Science and Innovation (MCI, PID2019- 109253RB-I00) and the Asturian regional government (FC-GRUPIN-IDI/2018/000181) are gratefully acknowledged. J. A.-V. also thanks the Asturian regional government for a predoctoral fellowship inside the Severo Ochoa programme (BP17-32). Prof. Wolfgang Kroutil (University of Graz, Austria) is acknowledged for the donation of overexpressed alcohol dehydrogenases and amine transaminases.

Financial support from the Spanish Ministry of Economy and Competitiveness(MEC,Project CTQ2016-75752-R) and the Asturian regional government (FC-GRUPIN-IDI/2018/000181) is gratefully acknowledged. J.A.- V. also thanks the Asturian regional government for a predoctoral fellowship in the Severo Ochoa programme. Prof. Wolfgang Kroutil (Universityof Graz,Austria) is gratefully acknowledged for the donation of overexpressed amine transaminases.

Financial supports from the Spanish Ministry of Economy and Competitiveness (MEC,Project CTQ2016-75752-R) and the Asturian Regional Government(FC-GRUPIN-IDI/2018/000181)are gratefully acknowledged.

Financial support from the Spanish Ministry of Economy and Competitiveness (MEC, Projects CTQ2013-44153-P and CTQ2016-75752-R) and the Government of the Principado de Asturias (Project FC-15-GRUPIN14-002) is gratefully acknowledged. A.M.-I thanks MEC for a predoctoral fellowship inside the FPI program, while L.A.Z thanks the national Brazilian agency CAPES for a postdoctoral fellowship

Spanish Ministry of Economy and Competitiveness (grant number: CTQ2016-75752-R); Spanish Ministry of Science and Innovation (grant number: PID2019-109253RB−I00); Asturian Regional Government (FC-GRUPIN-IDI/2018/000181)

Financial support from MICINN (Project CTQ2013-44153-P and CTQ2016-75752-R) and the Regional Government from the Principado de Asturias (Project FC-15-GRUPIN14-002) are gratefully acknowledged. L.M.-M. thanks the Regional Government from the Principado de Asturias for her predoctoral Severo Ochoa fellowship.

Financial support from the Spanish Ministerio de Economia y Competitividad (Projects CTQ2013-44153-P and CTQ2016-75752-R) and the Government of Principado de Asturias (Project FC-15-GRUPIN14-002) is gratefully acknowledged

The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under Grant Agreement No. 238531 (BIOTRAINS Marie Curie Initial Training Network). Financial support from the Spanish MICINN (MICINN-12-CTQ2011-24237) is also gratefully acknowledged. I.L. acknowledges MICINN for his research contract under the Ramón y Cajal Program

A.C. thanks the Principado de Asturias for his predoctoral fellowship Severo Ochoa. I. L. (Ramón y Cajal Program) thanks the Spanish MICINN for personal funding. Financial support 10 from MICINN (Project MICINN-12-CTQ2011-24237) and BIOTRAINS Marie Curie ITN, financed by the European Union through the Seventh Framework People Programme (Grant Agreement no. 238531) are gratefully acknowledged

The research for this work has received funding from the Eu-ropean Union (EU) project ROBOX (grant agreement nº635734)under EU's Horizon 2020 Program Research and Innovation actions H2020-LEIT BIO-2014-1. The views and opinions expressed in thisarticle are only those of the authors and do not necessarily reflectthose of the European Union Research Agency. The European Unionis not liable for any use that may be made of the informationcontained herein. Financial support from the Spanish Ministry of Economy and Competitiveness (MEC, Projects CTQ2013-44153-Pand CTQ2016-75752-R) is gratefully acknowledged. A.M.-I thanksMEC for a predoctoral fellowship inside the FPI program.

This project is supported by the BIOTRAINS Marie Curie Initial Training Network, financed by the European Union through the 7th Framework People Programme (grant agreement number 238531). Financial support from the Spanish MICINN (MICINN- 12-CTQ2011-24237) is also gratefully acknowledged. I.L. acknowledges MICINN for his research contract under the Ramón y Cajal Program

The chemical synthesis of a series of lignin-derived nitrogenated compounds was performed in high yields (73–99%) through simple conventional chemical transformations starting from natural monomers such as vanillin, syringaldehyde or 3,4-dihydroxybenzaldehyde. The study of the vanillin-derived compounds as substrates for commercially available laccases from Trametes versicolor and Myceliophthora thermophila in oxidative transformations, generally led to the isolation of several dimeric species in high to excellent conversions (>70%), while for hydrazone derivatives a more rapidly oxidative coupling was evidenced by the formation of oligomers and/or polymers. Remarkably, vanillin was obtained due to the hydrolysis of some of the nitrogenated functional groups, such as the hydrazone or the hydrazono tetrazole. The three families of lignin-derived compounds can provide a great source of new laccase-mediator systems (LMS), the possibility of employing them for lignin modification being particularly attractive. Preliminary experiments showed promising levels of activity towards the oxidation of a monomer (veratryl alcohol, up to 70% conversion) and a dimer (adlerol, up to 22% conversion) lignin models, higher than those achieved with the natural vanillin and syringaldehyde (up to 7% conversion with veratryl alcohol and almost negligible conversion with adlerol), these processes being also highly influenced by the pH of the reaction medium ; This work was supported by the Spanish Ministry of Economy and Competitiveness and the European Union (Project OXYPOL, PCIN-2014-134 from an ERA-NET ERA-IB 2 Call)

This research is part of BIONEXGEN project (grant agreement 266025) sponsored by the European Union inside the 7th Framework Programme (FP7 2007-2013). Financial support from MICINN (Project MICINN-12-CTQ2011-24237)is also gratefully acknowledged. We thank CLEA Technologies and Lentikats for providing some of the screened oxidases. L. M.-M. thanks the Principado de Asturias for her predoctoral fellowship Severo Ochoa. I.L. thanks the Spanish MICINN for personal funding (Ramón y Cajal Program).

The first report of a biocatalytic regioselective oxidative monocleavage of dialkenes was successfully achieved employing a cellfree enzyme preparation from Trametes hirsuta at the expense of molecular oxygen. Selected reactions were performed on a preparative scale affording high to excellent conversions and chemoselectivities ; This project is supported by the BIOTRAINS Marie Curie Initial Training Network, financed by the European Union through the 7th Framework People Programme (grant agreement number 238531). Financial support from the Spanish MICINN (Project CTQ-2007-61126) is also gratefully acknowledged. I.L. and V.G.-F. acknowledge MICINN for their research contracts under the Ramo´ n y Cajal Program