Suchergebnisse

Cyclamate is widely used as intense sweetener in the European Union. The absence of a maximum limit for the use of cyclamate in tabletop sweeteners and the growing demand for this type of product highlights the importance of developing robust analytical methods for the determination of its content to understand if the consumption of tabletop sweeteners can have a negative impact on human health. The present work aimed at the optimisation and validation of an high-performance liquid chromatography (HPLC) analytical method for cyclamate determination in tabletop sweeteners based on the procedure of European Standard EN 12857. The validated method was then applied to the determination of this sweetener in different types of tabletop sweeteners (liquid, powder and tablets). Both standards and samples solutions were submitted to a derivatisation procedure which converted cyclamate to N,N-dichlorocyclohexylamine. The derivatised product was separated and quantified using a reversed-phase column, a mobile phase composed of water (20%) and methanol (80%), isocratic flow of 1mlmin(-1), and detection by ultraviolet spectrophotometry at a wavelength of 314nm. The analytical method was internally validated according to the following validation parameters: working range, linearity, limits of detection and quantification, sensitivity, precision (repeatability and intermediate precision), and uncertainty. This method proved to be specific and selective for the determination of this sweetener, showing repeatability, RSDr 3%, intermediate precision, RSDR 3.3%, and recovery rates from 92% to 108% for the different tabletop sweeteners. The method uncertainty was 9.4%. The concentration of cyclamate in the samples varied significantly, from 2.9% to 73.9%, which demonstrated that a possible excessive consumption of one of the analysed sweeteners can lead to exceeding the acceptable daily intake for cyclamate. ; info:eu-repo/semantics/publishedVersion

"Intergovernmental Relations Subcommittee"--Page 1. ; Includes bibliographical references. ; CIS Index 1970 ; Mode of access: Internet.

International audience ; In 2005, 76 samples of non-alcoholic beverages were found to contain the intense sweeteners cyclamate, acesulfame-K, aspartame and saccharin out of 177 analysed samples. The content of cyclamate did in no cases exceed the now permitted maximum level in the European Union (EU) of 250 mgl-1 in soft drinks. The estimated intake of the sweeteners was calculated using the Danish Dietary Survey based on 3098 persons aged 1-80 years. The estimated intake with 90th percentiles of 0.7, 0.8 and 0.2 mgkg-1body weight (bw)day-1 for acesulfame-K, aspartame and saccharin , respectively, was much lower than the acceptable daily intake (ADI) values of 15, 40, 7 and 2.5 mgkg-1bwday-1 for acesulfame-K, aspartame and saccharin, respectively and on the same level as in the similar investigation from 1999. In contrast to the 1999 investigation, the 90th percentile of the estimated cyclamate intake in 1-3 year olds with 3.7 mgkg-1bwday-1 was in 2005 lower than the ADI value of 7 mgkg-1bwday-1. However, the 99th percentile for 1-3 year olds with 7.4 mgkg-1bwday-1 still exceeded the ADI value slightly. The 90th percentile for the whole population with 0.9 mgkg-1bwday-1 was halved compared with 1999. The reduction in EU of the maximum permitted level for cyclamate from 400 to 250 mgl-1 has brought the intake of cyclamate in small children down well below the ADI value.

Hazardous Chemicals -- Contents -- Figures and Tables -- Acknowledgments -- Abbreviations -- Introduction. A Conceptual and Regulatory Overview, 1800-2000 -- Part I. From Acute to Chronic Poisoning -- Chapter 1. Schweinfurt Green and the Sanitary Police -- Chapter 2. The Banning of White Lead -- Chapter 3. Old Situations, New Complications -- Part II. Discovering New Health Impacts -- Chapter 4. Discovering Chemical Carcinogenesis -- Chapter 5. Cyclamates -- Chapter 6. Cadmium Poisoning in Japan -- Chapter 7. Dioxins -- Part III. New Products, New Effects -- Chapter 8. Organophosphates -- Chapter 9. A Tale of Two Nations -- Chapter 10. War and Peace -- Chapter 11. Raising a Stink -- Conclusion -- Index.

We studied the eff ect of stevia leaf extract, saccharinate and cyclamate in various dosages on the glycogen content of blood leukocytes in mice, as a necessary substrate for the realization of phagocytosis. An increase of glycogen content was found in all experimental groups which obtained sweeteners at a dosage of 10 mg/g of body weight. The eff ect of sweeteners on the diff erential leukocyte counts was found only for mice treated with stevia leaf extract at a dosage of 10 mg/g of body weight. They demonstrated increase of neutrophil-to-lymphocyte ratio. New data expand our understanding of the metabolism of sugar substitutes in the organism and their eff ect on physiological systems, in particular the hematopoietic and immune systems.

Based on EC regulation 1333/2008, each member of the European Union is supposed to evaluate the intake of food additives by its population. This project resulted in a demand of the Federal Department of Public Health, Safety of the Food Chain and Environment to the Scientific Institute of Public Health to elaborate a study of table-top sweeteners and the estimation of the total intake of sweeteners by the Belgian population. The use of the selected sweeteners, including acesulfame-K, saccharin, cyclamate, aspartame and sucralose, is regulated by the European directive on sweeteners (94/35) and for each substance an acceptable daily intake (ADI; acceptable daily intake) was established (9, 5, 7, 40 and 15 mg per kg body weight). The purpose of this study was to establish whether the Belgian adult population was at risk of exposure to sweetener levels that exceeded ADI levels by usual intake of sweetened foods and specific consumption of table top sweeteners. ; BE; nl; joris.vanloco@wiv-isp.be

Los edulcorantes son aditivos alimentarios habituales en una gran variedad de bebidas, en particular de las bebidas carbonatadas y aromatizadas, denominadas comúnmente gaseosas. En la bibliografía se pueden consultar las concentraciones medidas de algunos edulcorantes en diversos productos alimentarios, pero no hay datos sobre las cantidades presentes en las bebidas carbonatadas, cantidades que tienen unos límites máximos legislados. En este contexto, se ha evaluado en este trabajo la posibilidad de determinar 4 edulcorantes y el ácido cítrico, aditivo habitual en este tipo de bebidas, mediante HPLC en fase inversa con una columna cromatográfica ODS y un detector convencional, y mediante dos técnicas espectroscópicas: absorción UV-visible y espectrofluorimetría. La preparación de la muestra es sencilla, solo se debe desgasificar y diluir las muestras. Así, se ha comprobado que la sucralosa no se puede determinar mediante estas técnicas debido a su muy baja absorción de radiación UV, mientras que la sacarina y el acesulfamo K pueden ser determinados mediante ambos tipos de técnicas. El ciclamato y el ácido cítrico no han podido ser determinados mediante HPLC ni de forma selectiva mediante las técnicas espectroscópicas, por lo que se ha propuesto un método de calibración de adiciones patrón para su determinación mediante espectroscopía UV-visible. La concentración de sacarina, acesulfamo K, ciclamato y ácido cítrico ha sido determinada en 4 muestras de gaseosa de diferente marca comercial. ; Sweeteners are common food additives in a wide variety of beverages, such as carbonated and flavored beverages, commonly referred to as soft drinks. In the literature, the measured concentrations of some sweeteners in various food products can be consulted, but there is no data on the amounts in carbonated beverages, which have legislated maximum limits. In this context, in this work, we evaluate the possibility of determining 4 sweeteners and citric acid, a common additive in this beverages, by HPLC in reverse phase with an ODS chromatographic column and conventional detectors, and by two spectroscopic techniques: UV-Vis absorption and spectrofluorimetry. Sample preparation is very simple, we just need to sonicate and dilute the samples. Thus, it has been found that sucralose cannot be determined by these techniques due to its very low absorption of UV radiation, while saccharin and acesulfame K can be determined by both types of techniques. Cyclamate and citric acid have not been able to be determined by HPLC or selectively by spectroscopic techniques, therefore a calibration method of standard additions has been proposed for their determination by UV-Vis spectroscopy. The concentration of saccharin, acesulfame K, cyclamate and citric acid has been determined in 4 soda samples of different commercial brands. ; Departamento de Química Analítica ; Máster en Técnicas Avanzadas en Química. Análisis y Control de Calidad Químicos

The prevalence of obesity among Chilean adults and children is one of the highest worldwide. To fight the constant increase of non-communicable diseases and the growing sales of sugar-sweetened beverages, the Chilean government recently enacted a new Law of Food Labeling and Advertising imposing the application of front-of-package warning labels in foodstuffs whose composition exceeds limits for critical nutrients including sugar. Accordingly, food companies have been reformulating their products, incorporating non-caloric sweeteners (NCSs) in partial or total replacement of sucrose. The number of NCS-containing foods and beverages, therefore, has been increasing in the last years. This study aims to identify the NCS-containing products from different food/beverage categories currently available on the Chilean market. Nineteen supermarkets and 13 food web pages were visited by trained dietitians to carry out a systematic search of ingredient information from the different food categories. Overall, 1,489 products were analyzed, of which 815 (55.5%) contained at least one NCS, being this proportion particularly high, compared to other countries. 67.1% of the dairy products, 31.5% of the cereal products, 49% of the processed fruits, 74.3% of the non-alcoholic beverages, and 46.2% of sweets and other desserts contained NCS. Considering the food categories more specifically oriented to children, NCSs were present in 98.8% of powder juices, 98.3% of the flavored milks, 91.2% of jellies, and 79% of the dairy desserts. Sucralose and steviol glycosides were the most widely used NCSs, these sweeteners being present, alone or mixed with other, in 73.5 and 39.7% of the NCS-containing products, respectively, while the use of saccharin and cyclamate was low. In addition, 80 tabletop NCSs were available in the local market, 91.2% of them being sucralose and steviol glycosides (alone or combined). The high number of food products containing steviol glycosides makes very plausible that the daily consumption of this NCS in the ...

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) ; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) ; No Brasil, muitas frutas, pouco exploradas economicamente, apresentam potencial como matéria-prima para a agroindústria. Este trabalho objetivou elaborar e determinar as características físico-químicas e sensoriais da geleia light de jambolão. Esta fruta tem uma coloração roxa intensa que conferiu às geleias, tanto padrão quanto light, aspecto visual bastante atrativo. As geleias light apresentaram características físico-químicas próximas daquelas desenvolvidas de forma convencional e, nas proporções de edulcorantes empregadas, os valores calóricos das formulações foram reduzidos na faixa de 41% a 53 %, enquadrando-se nas normas exigidas pela legislação brasileira para este tipo de produto. O perfil sensorial obtido para as 4 formulações light desenvolvidas evidenciou a preferência dos provadores pela geleia elaborada com a associação de ciclamato e sacarina. Assim, os resultados obtidos evidenciam boas perspectivas para a aplicação desta fruta na indústria de alimentos. ; In Brazil, several little economically explored fruits have good potential as raw material for the agro-industry. This study aimed to produce and determine the physical-chemical and sensory characteristics of light jambolan jelly. This fruit has intense purple color, which gave the jellies - both standard and light - a quite attractive visual aspect. The light jellies exhibited similar physical-chemical characteristics to the ones developed through the conventional method and; with the proportion of sweeteners used, the caloric values of the formulations were reduced to the range of 41 to 53%, attending the requirements of the Brazilian legislation for this type of product. The sensory profile obtained for the 4 light formulations developed, clearly showed the tasters' preference for the jelly elaborated with the association of cyclamate and saccharin. Thus, the results revealed good perspectives for the application of this fruit in the food industry.

The prevalence of obesity among Chilean adults and children is one of the highest worldwide. To fight the constant increase of non-communicable diseases and the growing sales of sugar-sweetened beverages, the Chilean government recently enacted a new Law of Food Labeling and Advertising imposing the application of front-of-package warning labels in foodstuffs whose composition exceeds limits for critical nutrients including sugar. Accordingly, food companies have been reformulating their products, incorporating non-caloric sweeteners (NCSs) in partial or total replacement of sucrose. The number of NCS-containing foods and beverages, therefore, has been increasing in the last years. This study aims to identify the NCS-containing products from different food/beverage categories currently available on the Chilean market. Nineteen supermarkets and 13 food web pages were visited by trained dietitians to carry out a systematic search of ingredient information from the different food categories. Overall, 1,489 products were analyzed, of which 815 (55.5%) contained at least one NCS, being this proportion particularly high, compared to other countries. 67.1% of the dairy products, 31.5% of the cereal products, 49% of the processed fruits, 74.3% of the non-alcoholic beverages, and 46.2% of sweets and other desserts contained NCS. Considering the food categories more specifically oriented to children, NCSs were present in 98.8% of powder juices, 98.3% of the flavored milks, 91.2% of jellies, and 79% of the dairy desserts. Sucralose and steviol glycosides were the most widely used NCSs, these sweeteners being present, alone or mixed with other, in 73.5 and 39.7% of the NCS-containing products, respectively, while the use of saccharin and cyclamate was low. In addition, 80 tabletop NCSs were available in the local market, 91.2% of them being sucralose and steviol glycosides (alone or combined). The high number of food products containing steviol glycosides makes very plausible that the daily consumption of this NCS in the pediatric populations could exceed its acceptable daily intake (ADI). The fact that there are no NCS-free foods alternatives for certain food categories, especially for children, is worrying. ; National Commission for Scientific and Technological Research CONICYT-FONDEF's National Fund for Research and Development in Health (Fondo Nacional de Investigacion y Desarrollo en Salud, FONIS) 2018 SA18I0062