Azulene-Derived Fluorescent Probe for Bioimaging:Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy
In: Murfin , L C , Weber , M , Park , S J , Kim , W T , Lopez-Alled , C M , McMullin , C L , Pradaux-Caggiano , F , Lyall , C L , Kociok-Köhn , G , Wenk , J , Bull , S D , Yoon , J , Kim , H M , James , T D & Lewis , S E 2019 , ' Azulene-Derived Fluorescent Probe for Bioimaging : Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy ' , Journal of the American Chemical Society , vol. 141 , no. 49 , pp. 19389-19396 . https://doi.org/10.1021/jacs.9b09813
Abstract
Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 665992
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