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In order to analyze the empirical instabilities and adopt new validation procedures for the Organizational Citizenship Behavior—Knowledge Workers (OCB-KW) Scale this study was organized in two stages. In the first stage, studies were read for a general characterization of the production of works that used the OCB-KW and from these data, it was possible to characterize applications of the scale and assess their possible instabilities. In the second stage of analysis, a secondary database was used and subjected to the parallel analysis, and then to the exploratory factor analysis for the initial estimation and validation of the constructs, and in a third moment, the confirmatory estimation of the constructs and the comparison of different models. From the comparison indices between models, it can be concluded that the best structure for the Organizational Citizenship Behavior—Knowledge Workers Scale is built from five dimensions correlated with each other.