BIOPOLYMER CONTROLLED RELEASE SYSTEMS BASED ON HYDROLYZED COLLAGEN: RELATIONSHIP OF CRYOFORMING CONDITIONS, STRUCTURE AND PROPERTIES
In: Vestnik Moskovskogo Universiteta Serija Chimija: Moscow University chemistry bulletin, Volume 65, Issue №3, 2024, p. 209-217
Biopolymer materials based on natural collagen (gelatin and hydrolyzed collagen) are widely used in the food, pharmaceutical and cosmetic industries due to their low toxicity, high biocompatibility, low antigenicity, and unique mechanical and technological properties. Hydrolyzed collagen, unlike gelatin, is formed by peptides with a lower molecular weight. It has higher bioavailability and biodegradability compared to gelatin. In this work, using low-temperature technologies, biopolymer matrices based on hydrolyzed collagen containing the antibacterial drug dioxidine were obtained. It has been shown that by varying such synthesis parameters such as the concentration of hydrolyzed collagen in the precursor solution (from 1 to 10%), matrix cross-linking time (0.1-24 hours), cryoforming temperature (-30 and -196 °C) it is possible to change the morphology and structure matrix, its degradation time and drug release time. The composition and structure of dioxidine/hydrolyzed collagen systems were characterized by SEM, IR and UV spectroscopy. The antibacterial activity of the resulting dioxidine/hydrolyzed collagen systems against E. coli and S. aureus was characterized by the disk diffusion method.