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The Maritime Situational Awareness Workshop (MSAW) was held 08 - 10 October 2019 in Lerici, La Spezia (Italy). CMRE organized this workshop with the objective of fostering the crossfertilization of ideas from scientific and military domains, toward the design and implementation of future solutions tailored to Maritime Situational Awareness (MSA) operational needs. Centred on the theme of Science and technology meet operational need, the call for papers was distributed amongst the international MSA community during the early months of 2019. In response, CMRE received 48 submissions and several contributions from the European Commission (EC) funded MSA projects. The workshop brought together about 170 participants from 23 countries including scientists, engineers, researchers from scientific communities with national and international authorities, end users and operators, and industrial representatives. Overall the audience was composed of 14% academia, 39% applied research institutes, 31% industry and 16% operational community. This document contains the proceedings of the MSAW'19 papers accepted for presentation. The MSAW'19 was co-sponsored by the EC project RANGER (Radars Long Distance Maritime Surveillance and SAR Operations) and NATO Allied Command Transformation as part of the CMRE Data Knowledge Operational Effectiveness programme. ; NATO Allied Command Transformation (ACT) EC project RANGER

© 2021 The Authors. ; The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers. ; Hyun-Dong Chang is supported by the Dr. Rolf M. Schwiete Foundation. Susanne Melzer and Attila Tarnok thank De Novo Software for providing FCS Express. Enrico Lugli is supported by a grant from the Associazione Italiana per la Ricerca sul Cancro (AIRC IG20676). Gabriele De Simone and Giovanni Galletti were supported by Fellowships from the Fondazione Italiana per la Ricerca sul Cancro-Associazione Italiana per la Ricerca sul Cancro (FIRC-AIRC). Jun Dong is supported by Deutsche Forschungsgemeinschft (DFG, German Research Foundation) Projektnummer 389687267 and Chinesisch-Deutsches Zentrum für Wissenschaftsförderung [Sino-German Center for Research Promotion (SGC)] grant C-0072. Nicola Gagliani, Samuel Huber and Franziska Muscate are supported by DFG fundings: SFB841,GA 2441/3-1, HU 1714/10-1. The tetramer APC-conjugated H-2K (d) HIV-1 gag197-205 AMQMLKETI used in TDS assay for mouse blood T cells was obtained through the NIH Tetramer Facility. Larissa Nogueira Almeida was supported by DFG research grant MA 2273/14-1. Supported by the following grants: AIRC 5X1000 2018 id. 21147 (Lorenzo Moretta); AIRC IG 2017 id. 19920 (Lorenzo Moretta); RC-2020 OPBG (Lorenzo Moretta); AIRC and European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 800924 (Linda Quatrini). Dirk Baumjohann was supported by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Emmy Noether Programme BA 5132/1-2 (252623821) and Germany's Excellence Strategy EXC2151 (390873048).