Suchergebnisse

[EN] NADH is a key biomolecule involved in many biocatalytic processes as cofactor and its quantification can be correlated to specific enzymatic activity. Many effort s have been t aken to obt ain clean electrochemical signals related to NADH presence and lower its redox overpotential to avoid interferences. Suppression of background and secondary signals can be achieved by including a switchable electroactive surface, for instance, by using semiconductors able to harvest light energy and drive the excited electrons only when irradiated. Here we present the combination of a n-type Si semiconductor with fibers made of carbon nanotubes as electroactive surface for NADH quantification at low potentials only upon irradiation. The resulting photoelectrode responded linearly to NADH concentrations from 50 μM to 1.6 mM with high sensitivity (54 μA cm −2 mM −1 ). This system may serve as a biosensing platform for detection and quantification of dehydrogenases' activity. ; European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 713366 . A.D.L., M.R and J.J.V. thank the "Comunidad de Madrid "for its support to the FotoArt-CM project ( S2018/NMT-4367 ) through the Program of R&D activities between research groups in Technologies 2018, co- financed by European Structural Funds. J.J.V. is grateful for generous financial support provided by the European Union Horizon 2020 Program under grant agreement 678565 (ERC-STEM) and by the MINECO ( RyC-2014-15115 HYNANOSC RTI2018-099504-A-C22 ; Peer reviewed