Gender Violence within the Inter American System of Human Rights Protection. The Resurgence of Gender Cultural Construct
In: Logos Universality Mentality Education Novelty: Political Sciences and European Studies, Band III, Heft 1, S. 49-65
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In: Logos Universality Mentality Education Novelty: Political Sciences and European Studies, Band III, Heft 1, S. 49-65
In: Romanian Journal of Military Medicine, Band 126, S. 3-11
ISSN: 2501-2312
"Chemotherapy is an important treatment in oncological disease, with a vast number of side effects. The cardiotoxicity of several chemotherapeutic agents and appropriate risk stratification and patient follow-up must be ensured by a multidisciplinary team which must include an oncologist and a cardiologist. Lynch syndrome is associated with younger-onset malignant tumors of various localizations, requiring aggressive chemotherapy. FOLFOX chemotherapy which is frequently used in Lynch syndrome-associated colorectal cancer has several cardiotoxic effects with mechanisms ranging from increased reactive oxidative species to Krebs cycle blockade or coronary vasospasm. These complex effects on the cardiovascular system have varied clinical effects, such as heart failure, arrhythmias, or acute ischemic events"
In: Romanian Journal of Military Medicine, Band 127, Heft 5, S. 341-350
ISSN: 2501-2312
Background: Head and neck cancers, and particularly, oral cancers have a complex pathogenesis that includes genetic
mutations and epigenetic alterations which interfere with cellular signaling and can trigger tumor development. The purpose of
this study was to reveal whether low-frequency hotspot mutations may be detected in a study lot with histopathological evidence
of squamous cell carcinoma (SCC) of the oral mucosa and skin of the head and neck. Methods: Tumor biopsies from treatment
naïve patients were tested for BRAF V600, NRAS G12/G13, NRAS Q61, KRAS Q61 mutations, and EGFR exon 19 deletions (Ex19Del)
using droplet digital PCR (ddPCR). The tumors were also analyzed for EGFR T790M mutations by RT-PCR, using a CE-IVD validated
kit, with a limit of detection of 0.05%. Results: None of the examined cases exhibited NRAS G12/G13, NRAS Q61, KRAS Q61, BRAF
V600, or EGFR T790M mutations, indicating that these alterations are rare events in SCC pathogenesis. Interestingly, among the
12 specimens tested by ddPCR for EGFR Ex19Del, an HPV-negative cSCC tumor occurring in the parotid region tested positive for
this drug-sensitizing mutation, offering unexplored therapeutic perspectives to the patient from whom it was collected.
Conclusions: Our study highlights the important clinical implications of detecting low-frequency hotspot mutations in tumor
biopsies by ddPCR. We believe that the ddPCR-assisted analysis of these mutations in larger SCC cohorts may provide us with
mechanistic insights regarding their role in SCC pathogenesis and guide the development of novel therapeutic strategies for this
problematic disease.