Com o objetivo de observar os parâmetros estabelecidos pela Instrução Normativa 70 de 2003 do Ministério da Agricultura Pecuária e Abastecimento, juntamente com o Programa Nacional de Monitoramento da Prevalência e da Resistência Bacteriana em Frango, o qual prevê o monitoramento de Salmonella sp. em produtos de frangos resfriados, foram estudadas carcaças e miúdos comestíveis (fígados e coração), condenados ou não por pericardite/perihepatite, em 60 lotes de aves abatidos sob Sistema de Inspeção Federal no estado do Tocantins, entre agosto de 2010 a junho de 2011. Foram isoladas 26 amostras indicativas de Salmonella sp. em 11 lotes (18,33%), sendo mais de uma estirpe por tipo de amostra. Os sorovares de maior frequência foram o Enteriditis (38,46%; 10/26) e Mbandaka (19,23%; 5/26), ambos isolados de coração, fígado e carcaça. Quanto ao perfil de resistência aos antimicrobianos, foram testados 12 princípios farmacológicos e as amostras apresentaram-se diferenciadas em alguns aspectos do encontrado na literatura consultada, sendo a maioria sensível às tetraciclinas, porém apresentaram 100% de resistência a um ou mais principio ativo, principalmente para Sulfamethoxazole (30 mcg) e Amoxicilina/Ácido Clavulânico (30 mcg). Apesar da Salmonella sp. ter sido isolada em carcaças normais, os resultados encontram-se dentro do permitido pela legislação brasileira vigente para produtos que ainda não sofreram processo de congelação. No entanto, deve-se sempre estar atento às condições higiênicas e sanitárias dos processos de produção e processamento de alimentos, principalmente quanto às boas práticas de fabricação. ; The aim of this study was to evaluate the profile of antibiotic resistance in Salmonella isolated from the carcasses, livers and hearts of chickens slaughtered in the state of Tocantins, Brazil, as recommended by the Normative Instruction 70 of 2003 of the Ministry of Agriculture, Livestock and Food Supply and the National Monitoring Program Prevalence and Bacterial Resistance in Chicken. Carcasses, livers and hearts from chicken with or without pericarditis/perihepatitis were studied in 60 lots of poultry slaughtered under the Federal Inspection System in the state of Tocantins, Brazil between August 2010 and June 2011. Twenty-six indicative Salmonella sp. were isolated in 11 lots (18.33%). Different strains of Salmonella were isolated more than a kind of sample/lot. The most frequent serovar was Enteriditis (38.46%, 10/26), while the second was Mbandaka (19.23%, 5/26), both isolated from hearts, livers and carcasses. Regarding antimicrobial resistance, of 12 tested principal pharmacological agents, the samples appeared to be most sensitive to tetracyclines, but showed 100% resistance to one or more active principal agents, especially sulfamethoxazole (30 mcg) and amoxicillin/clavulanic acid (30 mcg). Although Salmonella sp. was isolated from normal carcasses, the results are within permitted levels for unfrozen products according to Brazilian legislation. However, one should always be aware of the hygienic and sanitary conditions of production processes and food processing, especially regarding good manufacturing practices.
The aim of this study was to evaluate the profile of antibiotic resistance in Salmonella isolated from the carcasses, livers and hearts of chickens slaughtered in the state of Tocantins, Brazil, as recommended by the Normative Instruction 70 of 2003 of the Ministry of Agriculture, Livestock and Food Supply and the National Monitoring Program Prevalence and Bacterial Resistance in Chicken. Carcasses, livers and hearts from chicken with or without pericarditis/perihepatitis were studied in 60 lots of poultry slaughtered under the Federal Inspection System in the state of Tocantins, Brazil between August 2010 and June 2011. Twenty-six indicative Salmonella sp. were isolated in 11 lots (18.33%). Different strains of Salmonella were isolated more than a kind of sample/lot. The most frequent serovar was Enteriditis (38.46%, 10/26), while the second was Mbandaka (19.23%, 5/26), both isolated from hearts, livers and carcasses. Regarding antimicrobial resistance, of 12 tested principal pharmacological agents, the samples appeared to be most sensitive to tetracyclines, but showed 100% resistance to one or more active principal agents, especially sulfamethoxazole (30 mcg) and amoxicillin/clavulanic acid (30 mcg). Although Salmonella sp. was isolated from normal carcasses, the results are within permitted levels for unfrozen products according to Brazilian legislation. However, one should always be aware of the hygienic and sanitary conditions of production processes and food processing, especially regarding good manufacturing practices. ; Com o objetivo de observar os parâmetros estabelecidos pela Instrução Normativa 70 de 2003 do Ministério da Agricultura Pecuária e Abastecimento, juntamente com o Programa Nacional de Monitoramento da Prevalência e da Resistência Bacteriana em Frango, o qual prevê o monitoramento de Salmonella sp. em produtos de frangos resfriados, foram estudadas carcaças e miúdos comestíveis (fígados e coração), condenados ou não por pericardite/perihepatite, em 60 lotes de aves abatidos sob Sistema de Inspeção Federal no estado do Tocantins, entre agosto de 2010 a junho de 2011. Foram isoladas 26 amostras indicativas de Salmonella sp. em 11 lotes (18,33%), sendo mais de uma estirpe por tipo de amostra. Os sorovares de maior frequência foram o Enteriditis (38,46%; 10/26) e Mbandaka (19,23%; 5/26), ambos isolados de coração, fígado e carcaça. Quanto ao perfil de resistência aos antimicrobianos, foram testados 12 princípios farmacológicos e as amostras apresentaram-se diferenciadas em alguns aspectos do encontrado na literatura consultada, sendo a maioria sensível às tetraciclinas, porém apresentaram 100% de resistência a um ou mais principio ativo, principalmente para Sulfamethoxazole (30 mcg) e Amoxicilina/Ácido Clavulânico (30 mcg). Apesar da Salmonella sp. ter sido isolada em carcaças normais, os resultados encontram-se dentro do permitido pela legislação brasileira vigente para produtos que ainda não sofreram processo de congelação. No entanto, deve-se sempre estar atento às condições higiênicas e sanitárias dos processos de produção e processamento de alimentos, principalmente quanto às boas práticas de fabricação.
Visceral leishmaniasis (VL) is expanding in the Brazilian territory. Dogs are considered an important urban reservoir; however, studies have demonstrated the presence of infected cats in some Brazilian states. This report aimed to describe a case of Leishmania (Leishmania) infantum infection in a two-month-old domestic feline from a Brazilian region with a high incidence of human visceral leishmaniasis. The analyzed samples were the cat's blood, conjunctiva, spleen, liver, popliteal, submandibular and mesenteric lymph nodes, skin, lung and kidney. The diagnostic methods were: parasitological examination, polymerase chain reaction (PCR) and an immunoflurescence antibody test (IFAT). All tissues were positive. The title obtained using the IFAT was 1:160. The animal was negative for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). This work addresses the first case of feline leishmaniasis in the state of Tocantins, and reveals data that may contribute to the knowledge of the disease, since it has been shown to be able to develop rapidly and fatally in kittens, with the ability to infect several tissues.
Toxoplasma gondii and Neospora spp. are protozoa that have a significant impact on animal health due to the diseases they cause in domestic and wild animals. The aim of the present study was to investigate the presence of antibodies against T. gondii and Neospora spp. in cats from northern Brazil. Serum samples were collected from 180 cats in the municipality of Araguaína, Tocantins and used to evaluate the presence of anti-T. gondii and anti-Neospora spp. antibodies using the indirect fluorescent antibody test, with a cutoff of 1:64 and 1:25, respectively. The association between infection and individual animal characteristics (age, sex, origin, breed, and clinical signs) was tested using univariate analysis, followed by multivariate logistic regression. We found that 48.3% (87/180) of the animals had anti-T. gondii (95% CI: 40.8%–55.90%) and 3.9% (7/180) had anti-Neospora spp. (95% CI: 1.6%–7.8%) antibodies. There was no association between age, sex, breed origin, clinical signs, and seropositivity for T. gondii. Cats of defined breeds were more likely to be infected by Neospora spp. (OR = 10.7). Therefore, we found a high rate of seropositivity for T. gondii and a high rate of occurrence of Neospora infections in cats from the Araguaína region. The exposure of the feline population to the studied coccidia indicates the need to monitor the feline population for these infections and underscores the importance of effective sanitary measures against such pathogens.