Suchergebnisse

In the frame of the Xf-actors project, we focus attention on signals involved in plantpathogen interaction to develop innovative and sustainable approaches to control Xylella fastidiosa, associated with the olive quick decline syndrome. Lipids, components of the plasma and intracellular membranes, provide different biological functions. During plants-pathogen interactions lipids have different roles, as pathogen perception, signal transduction and downstream defence responses. Different bacterial species display diverse membrane compositions and even the membrane composition of cells is not constant but depends on the environmental conditions. Oxidized fatty acids are an important class of signalling molecule especially related to stress responses. Recently, other authors reported that oxylipins have a regulation activity in motility, biofilm formation and virulence in Pseudomonas aeruginosa. In this study, we explore the oxylipins signals of X. fastidiosa CoDiRO CFBP8402 in pure culture grown at 25°C. Moreover, we exploit the lipids signals during the plant-pathogen interaction with the model plant N. tabacum "Petite Havana SR1" after artificial inoculation with CoDiRO. The analysis were performed by LC-MS/MS in dynamic MRM modality allowing quantification of oleic, linoleic and linolenic acid -derived oxylipins. The results showed the presence of oxidized fatty acids in X. fastidiosa CoDiRO strain in pure culture and in inoculated tobacco plants. ; Acknowledgment This work has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement N. 727987 "Xylella fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy XF-ACTORS".

Fusarium langsethiae is amongst the most recently discovered pathogens of small grains cereals. F. langsethiae is the main producer, in Europe, of T2 and HT-toxins in small grain cereals, albeit often asymptomatic; this makes its control challenging. The European Union (EU) is pushing hard on the use of biocontrol agents to minimize the use of fungicides and pesticides, which are detrimental to the environment and responsible for serious pollution of the soil and superficial water. In line with EU directives (e.g., 128/2009), here we report the use of protein fractions, purified from the culture filtrate of the basidiomycete Trametes versicolor, for controlling F. langsethiae. T. versicolor, a so-called medicinal mushroom which is applied as a co-adjuvant in oncology and other pathologies as a producer of biological response modifiers. In this study, the exo-proteome of T. versicolor proved highly efficient in inhibiting the growth of F. langsethiae and the biosynthesis of the T2 toxin. Results are promising for its future use as a sustainable product to control F. langsethiae infection in cereals under field conditions.