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Potato peel from a snack factory was successfully used as feedstock for biobutanol production. This lignocellulosic biomass was subjected to various physicochemical pretreatments under different conditions and finally an autohydrolysis pretreatment was selected. The fermentability of the hydrolysates was checked with six Clostridia strains. It was observed that C. saccharobutylicum DSM 13864 produced 2.1 g/L acetone, 7.6 g/L butanol and 0.6 g/L ethanol in 96 h (0.186 gB/gS), whereas C. saccharoperbutylacetonicum DSM 2152 generated 1.8 g/L acetone, 8.1 g/L butanol and 1.0 g/L ethanol in 120 h (0.203 gB/gS). Detoxification steps of the hydrolysate before fermentation were not necessary. ; This PDF document is a pre-print version of the manuscript that was subsequently peer-reviewed and accepted for publication by the journal New Biotechnology (Vol. 46, pages 54-60, year 2018), within the Special Issue ICEEM09. The final publication is available at Elsevier via https://doi.org/10.1016/j.nbt.2018.07.002. The present work has been performed as part of the H2020-LCE-2015 Waste2Fuels project (Sustainable production of next generation biofuels from waste streams - Waste2Fuels. GA - 654623), funded by the European Union's Horizon 2020 Research and Innovation Programme. MH-V is supported by a postdoctoral contract (DOC-INIA, grant number DOC 2013-010) funded by the Spanish National Institute for Agricultural and Food Research and Technology (INIA) and the European Social Fund. Authors thank R. Antón del Río, N. del Castillo Ferreras and G. Sarmiento Martínez for their technical help.

Corn stover can be efficiently transformed into biobutanol by using low acid concentrations during the physicochemical pretreatment, employing adequate Clostridium strains and performing a detoxification with reusable resins. A broth with 4.75 g/L acetone and 9.02 g/L butanol was obtained. Off-line gas stripping is efficient for butanol recovery from fermentation broths. ; This document is a pre-print version of the manuscript that was subsequently peer-reviewed and accepted for publication by the journal Renewable Energy (Vol. 148, pages 223-233, year 2020). The final publication is available at Elsevier via https://doi.org/10.1016/j.renene.2019.12.026. This work has been funded by the European Union's Horizon 2020 Research and Innovation Programme (Agrocycle, grant agreement 690142). MH-V is supported by a postdoctoral contract (DOC-INIA, grant number DOC 2013-010) funded by the Spanish National Institute for Agricultural and Food Research and Technology (INIA) and the European Social Fund.

Apple pomace was studied as a possible raw material for biobutanol production. Five different soft physicochemical pretreatments (autohydrolysis, acids, alkalis, organic solvents and surfactants) were compared. The pretreated biomass was subsequently subjected to a conventional enzymatic treatment to complete the hydrolysis. One of the hydrolysates was readily fermented by Clostridium beijerinckii CECT 508 in 96 h (3.55 g/L acetone, 9.11 g/L butanol, 0.26 g/L ethanol; 0.276 gB/gS yield; 91% sugar consumption). Therefore, it is possible to optimise pretreatment conditions of lignocellulosic apple pomace to reduce inhibitor concentrations in the final hydrolysate and perform successful ABE fermentations without the need of a detoxification stage. ; This document is a pre-print version of the manuscript that was subsequently peer-reviewed and accepted for publication by the journal Applied Microbiology and Biotechnology (Vol. 101, pages 8041-8052, year 2017). The final publication is available at Springer via http://dx.doi.org/10.1007/s00253-017-8522-z. The present work has been performed as part of the H2020-LCE-2015 Waste2Fuels project (Sustainable production of next generation biofuels from waste streams - Waste2Fuels; GA - 654623), funded by the European Union's Horizon 2020 Research and Innovation Programme. MH-V is supported by a postdoctoral contract (DOC-INIA, grant number DOC 2013-010) funded by the Spanish Agricultural and Agrifood Research Institute (INIA) and the European Social Fund. The authors thank Novozymes for kindly providing samples of their enzymes. Authors thank R. Antón del Río, N. del Castillo Ferreras and G. Sarmiento Martínez for their technical help.

Vine shoots (Vitis vinifera L.) were assessed as a feedstock for lactic acid production. Vine shoots were pretreated with acidic or alkaline solutions. The best pretreatment consisted in H2SO4 1.72% (w/w) at 134°C for 17 min, followed by an enzymatic hydrolysis. Three thermotolerant strains, namely Bacillus coagulans DSM 2314, Geobacillus stearothermophilus DSM 2313 and G. stearothermophilus DSM 494, were compared to produce lactic acid from vine-shoot hydrolysates under aerobic and non-sterile conditions, without the need of detoxification steps. In addition, wine lees were satisfactorily employed as nitrogen sources for the fermentation; providing similar results to yeast extract and being the only nutrient added to vine-shoot hydrolysates. Under optimal conditions, B. coagulans DSM 2314 produced 29.21 ± 0.23 g/L lactic acid in 24 h, with a sugar consumption of 98.74 ± 0.07 % and a yield of 96.38 ± 0.76 %, when supplemented with red wine lees. ; This study was funded by the European Regional Development Fund (ERDF) within the programme Interreg V-A España-Portugal (POCTEP) 2014-2020 through the project BIOVINO [0688_BIOVINO_6_E] and also by the H2020-WASTE-2015-two-stage Agrocycle project (Sustainable techno-economic solutions for the agricultural value chain. GA - 690142), funded by the European Union's Horizon 2020 Research and Innovation Programme. This preprint has not undergone peer review or any post-submission improvements or corrections. The Version of Record of this article is published in Applied Microbiology and Biotechnology (2021) 105, 9385-9402, and is available online at https://doi.org/10.1007/s00253-021-11693-1.