Suchergebnisse

This research was funded by Ministerio de Ciencia, Innovacion y Universidades under the "Proyectos de I+D+i « RETOS INVESTIGACION »" (grant number RTI2018-096724-B-C22); and by Junta de Andalucia, under the "Proyectos I+D+i del Programa Operativo FEDER 2018" (grant number B-AGR-466-UGR18), the "Ayudas a proyectos de I+D+I de entidades privadas calificadas como agentes del sistema andaluz del conocimiento" (grant number PY18-RE-0033), the "Ayudas al funcionamiento de los Grupos operativos de la Asociacion Europea para la Innovacion (AEI) en materia de productividad y sostenibilidad agricolas en el sector del olivar, 2020" (grant number GOPOGR-20-0001), and, finally, the "Ayudas a los agentes publicos del Sistema Andaluz del Conocimiento, para la realizacion de proyectos de I+D+i" (grant number A-AGR-226-AGR20). ; Olive leaves, one of the most abundant olive production by-products, have shown incredible potential for their characteristic bioactive compound composition, with unique compounds such as the polyphenol oleuropein. In order to evaluate the bioaccessibility of bioactive compounds present in an olive leaf extract, samples were submitted to an in vitro digestion process following INFOGEST protocol, and qualitative and quantitative characterization of the original extract and digestive samples at different times were carried out using HPLC-ESI-TOF-MS. The analyzed extract presented an abundance of phenolic compounds, such as secoiridoids, with oleuropein being the main identified compound. The in vitro digestion process showed an effect on the phenolic profile of the extract, with a lower recovery in the gastric phase and an increase at the beginning of the intestinal phase. Most of the studied compounds showed high bioaccessibility at the end of the digestion, with oleuropein, ligstroside, and quercetin-3-O-galactoside being among the ones with higher value. These findings show the potential for future use of olive leaf polyphenols. However, further research is needed in order to evaluate the absorption, delivery, and interaction of these compounds with the colon. ; Spanish Government RTI2018-096724-B-C22 ; Junta de Andalucia B-AGR-466-UGR18 ; Ayudas a proyectos de I+D+I de entidades privadas calificadas como agentes del sistema andaluz del conocimiento PY18-RE-0033 ; Ayudas al funcionamiento de los Grupos operativos de la Asociacion Europea para la Innovacion (AEI) en materia de productividad y sostenibilidad agricolas en el sector del olivar, 2020 GOPOGR-20-0001 ; Ayudas a los agentes publicos del Sistema Andaluz del Conocimiento, para la realizacion de proyectos de I+D+i A-AGR-226-AGR20

[Introduction]: Olea europaea L. leaves may be considered a cheap, easily available natural source of phenolic compounds. In a previous study we evaluated the possibility of obtaining bioactive phenolic compounds from olive leaves by pressurised liquid extraction (PLE) for their use as natural anti-oxidants. The alimentary use of these kinds of extract makes comprehensive knowledge of their composition essential. [Objective]: To undertake a comprehensive characterisation of two olive-leaf extracts obtained by PLE using high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS). [Method]: Olive leaves were extracted by PLE using ethanol and water as extraction solvents at 150°C and 200°C respectively. Separation was carried out in a HPLC system equipped with a C 18-column working in a gradient elution programme coupled to ESI-QTOF-MS operating in negative ion mode. [Results]: This analytical platform was able to detect 48 compounds and tentatively identify 31 different phenolic compounds in these extracts, including secoiridoids, simple phenols, flavonoids, cinnamic-acid derivatives and benzoic acids. Lucidumoside C was also identified for the first time in olive leaves. [Conclusion]: The coupling of HPLC-ESI-QTOF-MS led to the in-depth characterisation of the olive-leaf extracts on the basis of mass accuracy, true isotopic pattern and tandem mass spectrometry (MS/MS) spectra. We may conclude therefore that this analytical tool is very valuable in the study of phenolic compounds in plant matrices. Copyright © 2012 John Wiley & Sons, Ltd. ; This work was supported by the projects AGL2008-05108-C03-03/01 and AGL2011-29857-C03-02 of the Spanish Ministry of Science and Innovation; P09-CTS-4564, P10-FQM-6563 and P11-CTS-7625 of the Andalusian Regional Government Council of Innovation and Science, and GREIB.PT.2011.18. The authors are grateful to the Spanish Ministry of Science and Innovation for FPU grant AP2007-03246 and a 'Ramón y Cajal' research contract. ; Peer Reviewed

In an extensive search for bioactive compounds from plant sources, the composition of different extracts of rosemary leaves collected from different geographical zones of Serbia was studied. The qualitative and quantitative characterization of 20 rosemary (Rosmarinus officinalis) samples, obtained by microwave-assisted extraction (MAE), was determined by high performance liquid chromatography coupled to electrospray quadrupole-time of flight mass spectrometry (HPLC–ESI-QTOF-MS). The high mass accuracy and true isotopic pattern in both MS and MS/MS spectra provided by the QTOF-MS analyzer enabled the characterization of a wide range of phenolic compounds in the extracts, including flavonoids, phenolic diterpenes and abietan-type triterpenoids, among others. According to the data compiled, rosemary samples from Sokobanja presented the highest levels in flavonoids and other compounds such as carnosol, rosmaridiphenol, rosmadial, rosmarinic acid, and carnosic acid. On the other hand, higher contents in triterpenes were found in the extracts of rosemary from Gložan (Vojvodina). ; This work was financially supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia through a national project (Project Number TR 31014) and through scientific and technological cooperation between the Republic of Serbia and Spain (Project Number RE451-03-02635/2011-14/4). The authors are also grateful to the Spanish Ministry of Science and Innovation for the project AGL2011-29857-C03-02 and the grant FPI BES-2009-028128, Andalusian Regional Government Council of Innovation and Science for the Excellence Projects P09-CTS-4564, P10-FQM-6563 and P11-CTS-7625