Suchergebnisse

In recent years, food ingredients rich in bioactive compounds have emerged as candidates to prevent excess adiposity and other metabolic complications characteristic of obesity, such as low-grade inflammation and oxidative status. Among them, fungi have gained popularity for their high polysaccharide content and other bioactive components with beneficial activities. Here, we use the C. elegans model to investigate the potential activities of a Grifola frondosa extract (GE), together with the underlying mechanisms of action. Our study revealed that GE represents an important source of polysaccharides and phenolic compounds with in vitro antioxidant activity. Treatment with our GE extract, which was found to be nongenotoxic through a SOS/umu test, significantly reduced the fat content of C. elegans, decreased the production of intracellular ROS and aging-lipofuscin pigment, and increased the lifespan of nematodes. Gene expression and mutant analyses demonstrated that the in vivo anti-obesity and antioxidant activities of GE were mediated through the daf-2/daf-16 and skn-1/nrf-2 signalling pathways, respectively. Taken together, our results suggest that our GE extract could be considered a potential functional ingredient for the prevention of obesity-related disturbances. ; This work was performed with the financial support of the CIEN project BIOPRO from "Centro para el Desarrollo Tecnológico Industrial" (CDTI), Ministry of Science and Innovation, Government of Spain. The authors thank ARGAL ALIMENTACIÓN S.A., ANECOOP, ELMUBAS IBÉRICA S.L.U., Bodega Matarromera S.L., Kimitec Group, Mahou San Miguel, UVESA, and Entomo AgroIndustrial for their support of this project. We also thank Bolet Ben Fet (TEB Verd SCCL., Barcelona). Finally, the authors thank LABORATORIOS CINFA, S.A. and VISCOFAN S.A for the financial support of the Center for Nutrition Research, and the CERCA Programme (Generalitat de Catalunya) for the financial support of IRTA. ; Peer reviewed