Suchergebnisse

Mosquitoes are the most important arthropods from the point of view of public health, due to the fact that they can transmit a large number of pathogens which can cause diseases to humans and animals. Aedes aegypti (L.) is one of the most important vector species in the world, since it can transmit numerous pathogens such as dengue, Zika, and chikungunya. Therefore, studies involving the molecular aspects of this and other mosquitoes species are currently increasing. In this report, we describe the comparison between two DNA extraction techniques, Chelex and cetyltrimethylammonium bromide (CTAB), for carrying out DNA extraction in larvae, pupae and adult female of Ae. aegypti. The Chelex technique was superior in the amount and purity of DNA as compared to the CTAB technique in the three life stages we tested. ; Department for Environment Food and Rural Affair (DEFRA), Scottish Government [SV3045, SE4113]; Department for Environment Food and Rural Affair (DEFRA), Welsh Government [SV3045, SE4113]; EUEuropean Union (EU) [653316] ; Luis M Hernandez-Triana would like to thank the Department for Environment Food and Rural Affairs (DEFRA), Scottish Government and Welsh Government through grants SV3045, and SE4113, and the EU Framework Horizon 2020 Innovation Grant, European Virus Archive (EVAg, grant no. 653316) for funding.