Trehalose-based Janus cyclooligosaccharides: the ''Click'' synthesis and DNA-directed assembly into pH-sensitive transfectious nanoparticles
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.-- et al. ; The convergent preparation of Janus molecular nanoparticles by thiourea-''clicking'' of a,a0 -trehalose halves has been implemented; the strategy allows access to macrocyclic derivatives with seggregated cationic and lipophilic domains that in the presence of DNA undergo pH-dependent self-assembly into lamellar superstructures, as established by electrochemical, structural (SAXS), microscopical (TEM) and computational techniques, that mediate transfection in vitro and in vivo. ; We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). ; The authors thank MINECO (contract numbers CTQ2012- 30821, SAF2013-44021-R, CTQ2015-64425-C2-1-R and CTQ2015-64425-C2-2-R), the Junta de Andalucía (contract number FQM2012-1467), University Complutense of Madrid (project no. UCMA05-33-010), the Government of Navarra (Department of Innovation and Industry, contract number IIQ14334.RI1), the University of Navarra Foundation (FUN), and the European Regional Development Funds (FEDER and FSE) for financial support. ; Peer reviewed
