Suchergebnisse

The continuous monitoring of the relationship between land surface temperature (LST) and land cover change is imperative for an inquiry into the potential impact of LST on human well-being, including urban outdoor thermal comfort in Lagos State, Nigeria. Using Landsat imagery, this study assessed land cover and LST changes from 1984 to 2019. Land cover was extracted, LST was determined from Landsat imageries and the land cover changes were linked to LST using the contribution index (CI). Afterwards, the universal thermal climate index (UTCI) was calculated to determine the heat stress levels. Findings confirm the presence of urban sprawl and new growth areas in previously rural Local Government Areas (LGAs) eastward, northward and westward of Lagos metropolis and in regions which lie on the fringe of the state's border with Ogun State. Also, a very strong link between high LST intensities and increasing concentration of urban areas in rapidly growing LGAs has been observed. This link further confirms the increased warming of the state, with an increase in mean LST of 2.16°C during 1984–2019. The UTCI which was used to gauge the influence of LST on outdoor thermal comfort ranged from moderate to strong heat stress levels. This study helps to strengthen the case for definite policies and actions which should be aimed at achieving moderate urban development through increased urban tree canopy/green infrastructure provision and carbon sequestration activities in urban design/landscape design, in Lagos State.

BACKGROUND: Plasmodium falciparum-resistance to sulphadoxine-pyrimethamine (SP) has been largely reported among pregnant women. However, the profile of resistance markers to SP dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) in the general population are varied and not frequently monitored. Currently, SP is used as partner drug for artemisinin combination therapy (SP-artesunate) in some sub-Saharan African countries or as a prophylactic drug in intermittent preventive treatment of malaria during pregnancy and infants and in seasonal malaria chemoprevention (SMC). Profiling of P. falciparum-resistant genotypes to SP is dynamic and critical in providing data that would be useful for malaria control programmes. This study assessed the profile of dhfr and dhps genes genotypes among individuals with malaria in Lagos, Nigeria. METHODS: Molecular markers of SP resistance were identified by nested PCR and sequenced among malaria positive dried blood spots (DBS) that were collected from individuals attending health facilities from January 2013 to February 2014 and during community surveys from October 2010 to September 2011 across different Local Government Areas of Lagos State, Nigeria. RESULTS: A total of 242 and 167 samples were sequenced for dhfr and dhps, respectively. Sequence analysis of dhfr showed that 95.5% (231/242), 96.3% (233/242) and 96.7% (234/242) of the samples had N51I, C59R and S108N mutant alleles, respectively. The prevalence of dhps mutation at codons A437G, A613S, S436A, A581G, I431V and K540E were 95.8% (160/167), 41.9% (70/167), 41.3% (69/167), 31.1% (52/167), 25.1% (42/167), and 1.2% (2/167) respectively. The prevalence of triple mutations (CIRNI) in dhfr was 93.8% and 44.3% for the single dhps haplotype mutation (SGKAA). Partial SP-resistance due to quadruple dhfr-dhps haplotype mutations (CIRNI-SGKAA) and octuple haplotype mutations (CIRNI-VAGKGS) with rate of 42.6% and 22.0%, respectively has been reported. CONCLUSIONS: There was increased prevalence in dhfr triple ...