Suchergebnisse

Abstract: Stroke has a significant prevalence in Romania. The predisposition for this multifactorial disease is partially known. The aim of this study is to investigate the predisposition for stroke in Romanian population. Material and methods. We selected cases with recent ischemic stroke (n=100) and healthy control subjects (n=100). The AGTR1 A1166C (rs5186) polymorphism was genotyped by restriction of amplicons with Dde I endonuclease. Results. Active cigarette smoking (O.R. =6.92, p=0.0001) or presence of the AT1R C variant (O.R. =6.69, p=0.0006) in overweight or obese women significantly increase the risk for ischemic stroke. The diagnosis of stroke (71.5 vs 68 years old) or T2DM (63.39 vs 60.77 years old) was recorded at an older age in women compared to men (p<0.05). Obesity considered independent (O.R. =4.22, p<0.05) or in association with T2DM (O.R. = 10.16, p=0.0002) confers the highest risk of stroke when compared to women. Conclusions. Obesity in association with T2DM confers the highest risk of stroke for men when compared to women. Active cigarette smoking or AT1R C variant significantly increase the risk for stroke in women with a high BMI compared with controls. Keywords: stroke, AT1R, BMI, cigarette smoking

The long-distance post-SARS-CoV2 infection effects were partially known. The study aims to follow the evolution of the patients with acute post-COVID 19 clinical symptoms presented in the Emergency Department. Methods: We analyzed 262 presentations more than 14 days after the initial COVID-19 diagnosis and with at least one acute COVID-19-related symptom. This study included presented between 01.01 and 31.05.2021 in the Emergency Department of Bucharsti- Emergency University Hospital. Data were obtained in real-time, at the presentation, from the patients' medical charts, and afterward from SUUB's database. Results. Out of the total of 262 presentations, 40.84% required patient hospitalization. The highest number of presentations with (18.70%) or without (22.90%) hospitalization was recorded in the first month after the COVID-19 diagnosis. Patients with less than 30 days between presentations had significantly fewer hospitalization days, compared to those with more than 30 days between presentations (8.3 vs 14.14 days, p=0.009). We found a high amount of hospitalization days (1093 days), with 29.88% deaths. Conclusions: COVID-19 complications require prolonged hospitalization, with implications on the patients' quality of life, the overcrowding of intensive care facilities, high-cost treatment, and unfavorable evolution for a great number of patients

Different factors may be involved in the clinical heterogeneity of spinal muscular atrophy disease. The vitamin D receptor (VDR) might be a candidate gene for this disease. Our study aimed to assess the preferential transmission of VDR polymorphisms from parents to SMA children. We genotyped 261 subjects (87 SMA nuclear families) for VDR FokI, BsmI, ApaI, and TaqI polymorphisms. The transmission of the genetic marker was estimated with Plink and FBAT software. It detected a preferential transmission of the rs731236 and rs7975232 variants to SMA1 patients and of rs1544410 variants to SMA2 patients. The variants of rs2228570 were preferentially transmitted to parents of all SMA patients. Haplotype analysis identified that haplotypes C-C-G-A and T-A-A-G seem to be involved in the booth type of SMA whereas the impact of T-A-A-A seems to be limited only to SMA2. Strong linkage disequilibrium (LD) between rs7975232 and rs1544410 was detected in samples from parents. Even though we investigated a small number of nuclear families the results suggest a potential link between VDR polymorphisms and SMA disease.

"The cytogenetic aberrations induced by doxorubicin (DOX) may be influenced by diet. Investigation regarding effects of low doses of DOX on chromosomal aberrations frequency in mice bone marrow. The study explored a possible relationship between diet, follow-up period, and cytostatic doses. Four mice groups received normal or high-fat (pork) diet. At the beginning of the 8th day mice from each group received a single intraperitoneal injection with DOX (dose range: 1.7-7.7 mg/kg b.w.) or isotonic saline solution (0.02 mL/g control mice). In the 10th (groups I, II) and 14th (groups III, IV) days from the beginning of the experiment, the mice were euthanized and chromosome aberrations were tested in bone marrow cells. The body weight of all mice who received DOX decreases, especially at higher doses. In the first three days post-injection, weight loss percentage was significantly influenced by DOX doses and/or type of diet (p<0.003). The interaction between the type of diet and DOX doses was not statistically significant in the follow-up period (p interactions >0.05). DOX doses were able to increase the frequency of chromosomal aberrations. DOX acts as a potent inductor of cytogenetic aberrations in bone marrow cells, regardless of the type of diet."