Suchergebnisse

This study received funding from the Basque Country Government, within the Elkartek program, phase I. Support program for collaborative research in strategic area, within the project named SINET (reference KK-2018/00048).

This work received partial financial support from the Administrative Department of Science, Technology and Innovation (Colciencias), a government agency of the Republic of Colombia, within the project "Determination of the Prevalence of Myopia and its association with environmental influences in an urban and rural population in Colombia- MIOPUR study". Code 651756933785.

This paper was supported by grant 125|2012, Sociedad Española de Neumología y Cirugía Torácica" (SEPAR). Beatriz García is funded by the Government of the Principado de Asturias (Spain) and the consolidated research groups grant FC-15-GRUPIN14–141. The Instituto Universitario Fernández-Vega is supported in part by the Fundación de Investigación Oftalmológica through the Fundación Cristina Masaveu Peterson, Spain.

This study received funding from the Ministry of Economy and Competitiveness of the Spanish Government, within the project denominated SURFEYE (reference RTC2014-2375-1).

This research was supported by the grant PI17/01549 from the "Acción Estratégica en Salud (AES)"-Instituto de Salud Carlos III-of the Spanish Ministry of Economy and Competitiveness, and the European Union through the "Fondo Europeo de Desarrollo Regional (FEDER)".

This paper was supported by a grant from Sociedad Madrileña de Neumología y Cirugía Torácica (XV Edición Premios Neumomadrid). Carla Martin was funded by Allergan SA with the "Cátedra Allergan de Innovación en superficieocular" (CAT-15-ALLERGAN) and Beatriz García was fundedby the Government of the Principado de Asturias (Spain) and the consolidated research groups grant FC-15-GRUPIN14-141. The Instituto Universitario Fernández-Vega is supported in partby the Fundación de Investigación Oftalmológica through the Fundación Cristina Masaveu Peterson, Spain.

This work was supported in part by the Spanish Ministry of Economy, the INDREYE Project IPT-2012-0438-010000, the Fundación Ramón Areces, Spain, and the Fundación Cristina Masaveu-Peterson, Spain. Beatriz García is funded by the Government of the Principado de Asturias (Spain), through the consolidated research groups Grant FC-15-GRUPIN14-141

Acanthamoeba keratitis is a serious pathogenic corneal disease, with challenging diagnosis. Standard diagnostic methods include corneal biopsy (involving cell culture) and in vivo reflection corneal microscopy (in which the visualization of the pathogen is challenged by the presence of multiple reflectance corneal structures). We present a new imaging method based on fluorescence sectioned microscopy for visualization of Acanthamoeba. A fluorescent marker (MT-11-BDP), composed by a fluorescent group (BODIPY) inserted in miltefosine (a therapeutic agent against Acanthamoeba), was developed. A custom-developed fluorescent structured illumination sectioned corneal microscope (excitation wavelength: 488 nm; axial/lateral resolution: 2.6 μm/0.4-0.6 μm) was used to image intact enucleated rabbit eyes, injected with a solution of stained Acanthamoeba in the stroma. Fluorescent sectioned microscopic images of intact enucleated rabbit eyes revealed stained Acanthamoeba trophozoites within the stroma, easily identified by the contrasted fluorescent emission, size and shape. Control experiments show that the fluorescent maker is not internalized by corneal cells, making the developed marker specific to the pathogen. Fluorescent sectioned microscopy shows potential for specific diagnosis of Acanthamoeba keratitis. Corneal confocal microscopy, provided with a fluorescent channel, could be largely improved in specificity and sensitivity in combination with specific fluorescent marking. © 2012 Optical Society of America. ; The authors acknowledge funding from a CSIC Proyecto Intramural Frontera PIF80F0171/2/3; Ministerio de Ciencia e Innovación, Spain, FIS2008-02065 (S. M.), FIS2011-25637 (S. M.), RYC2006-2920 (J. R. I.), FIS2009-07966 (J. R. I.), CTQ2010-16457 (A. U. A.), PROFIT CIT-300100-2007-50 (J. M. L.); Instituto de Salud Carlos III ISCIII- (RICET)-FEDER RD 06/0021/0006 (L. R.), and FIS 2009-01928 (L. R.); European Union FP7 HEALTH-2007-223414 (L. R.); EURHORCS-ESF Grant EURYI-05-102-ES (S. M.). ; Peer Reviewed

Supported in part by Grants FIS PI11/02886, MINECO-13-IPT-2012-0438-010000, and MINECO-13-IPT-2012-1029-010000 from the Ministry of Economy and Competitiveness, Government of Spain, cosponsored with FEDER (Fondo Europeo de Desarrollo Regional) funds from the European Union; and Grant FC-15-GRUPIN14-141 from the Ministry of Economy and Employment of the Government of Principado de Asturias, Spain. BG is funded by the Government of Principado de Asturias (Spain), Consolidate Research Groups Grant FC-15-GRUPIN14-141. The Instituto Universitario Fernández-Vega is supported in part at Fundación de Investigación Oftalmológica by Fundación Cristina Masaveu Peterson, Spain.

Instituto de Salud Carlos III-Fondo de Investigacion Sanitaria [PI051564]; Government of the Principado de Asturias (Spain) [FC-15-GRUPIN14-141]

Acción Estratégica en Salud (AES), Instituto de Salud Carlos III, of the Spanish Ministry of Economy and Competitiveness [PI17/01549]; European Union through the Fondo Europeo de Desarrollo Regional (FEDER).

This work was supported in part by grants: MINECO-13-IPT-2012-0438-010000 and MINECO-13-IPT-2012-1029-010000, from the Ministry of Economy and Competitiveness, Government of Spain, co-sponsored with FEDER funds from the European Union; FC-15-GRUPIN14-141 from the Ministry of Economy and Employment of the Government of Principado de Asturias, Spain, and Allergan Chair of innovation in ocular surface at the University of Oviedo. BG is funded by the Government of the Principado de Asturias (Spain), consolidated research groups grant FC-15-GRUPIN14-141. The Instituto Universitario Fernández-Vega is supported in part by the Fundación de Investigación Oftalmológica through the Fundación Cristina Masaveu Peterson, Spain.

Funding: This work was supported by the grant PI17/01549 from the "Acción Estratégica en Salud (AES)"-Instituto de Salud Carlos III-of the Spanish Ministry of Economy and Competitiveness, and the European Union through the "Fondo Europeo de Desarrollo Regional (FEDER)".

This work was supported by Fundación Mutua Madrileña, Government of Asturias (FEDER-EU - FC-15-GRUPIN 14-141) and Telefónica