Suchergebnisse

Phytoterapic compounds have been used in wound healing for many centuries. Nowadays, scientific evidences of phytotherapeutics is a requirement of the legislation. The scientific literature notes the need for healing topics yielding scars that are both aesthetically appealing and resistant. We aimed to evaluate the cytotoxicity of several doses of T. aestivum extract (2 mg mL-1, 4 mg mL-1, 6 mg mL-1, 8 mg mL-1 and 10 mg mL-1) in a fibroblast cell line and the healing process in an in vivo experimental model (New Zealand rabbits). For this, MTT test in 3T6 cells was performed in duplicates using MEM (0 mg ml-1) as negative control. Cell viability was calculated as: absorbance average in treatments/absorbance average in controls x 100. In vivo test was performed in 78 skin wounds in rabbits that were treated with 2 mg ml-1and 10 mg ml-1 of T. aestivum and non-ionic cream for 21 days. After this period, it was evaluated the histology using picrosorius and Gomori's trichrome staining. Statistical analysis was evaluated using T test (Graphpad) for cytotoxicity assay, Fischer test for the gomori trichrome test (Grahpad) and Kruskal-Wallis (Statistic 9.0) for picrosirius test. The in vitro test resulted in cytotoxicity observed at 2mg mL-1 whereas cells were viable at higher doses. On the other hand, it was observed that collagen formation of wounds was more uniform with this dose than with 10mg mL-1 extract in the in vivo study. Thus, we conclude that the 2mg mL-1 T. aestivum aqueous extract dose was more efficient in the in vivo wound healing study, despite its cytotoxic effects in vitro.

Phytoterapic compounds have been used in wound healing for many centuries. Nowadays, scientific evidences of phytotherapeutics is a requirement of the legislation. The scientific literature notes the need for healing topics yielding scars that are both aesthetically appealing and resistant. We aimed to evaluate the cytotoxicity of several doses of T. aestivum extract (2 mg mL-1, 4 mg mL-1, 6 mg mL-1, 8 mg mL-1 and 10 mg mL-1) in a fibroblast cell line and the healing process in an in vivo experimental model (New Zealand rabbits). For this, MTT test in 3T6 cells was performed in duplicates using MEM (0 mg ml-1) as negative control. Cell viability was calculated as: absorbance average in treatments/absorbance average in controls x 100. In vivo test was performed in 78 skin wounds in rabbits that were treated with 2 mg ml-1and 10 mg ml-1 of T. aestivum and non-ionic cream for 21 days. After this period, it was evaluated the histology using picrosorius and Gomori's trichrome staining. Statistical analysis was evaluated using T test (Graphpad) for cytotoxicity assay, Fischer test for the gomori trichrome test (Grahpad) and Kruskal-Wallis (Statistic 9.0) for picrosirius test. The in vitro test resulted in cytotoxicity observed at 2mg mL-1 whereas cells were viable at higher doses. On the other hand, it was observed that collagen formation of wounds was more uniform with this dose than with 10mg mL-1 extract in the in vivo study. Thus, we conclude that the 2mg mL-1 T. aestivum aqueous extract dose was more efficient in the in vivo wound healing study, despite its cytotoxic effects in vitro. ; Os extratos vegetais têm sido utilizados na cicatrização de feridas a muitos séculos. No entanto nos dias atuais a comprovação científica dos fitoterápicos é uma exigência da legislação. Na literatura científica se observa a necessidade de cicatrizantes tópicos que proporcionem uma cicatriz estética e resistente. Devido a isso objetivou-se avaliar a citotoxicidade de diversas doses de T. aestivum (2 mg mL1, 4 mg mL-1, 6 mg mL-1, 8 mg mL-1 e 10 mg mL-1) em linhagem celular de fibroblasto, e o processo cicatricial em modelo experimental (New Zealand rabbits) in vivo. Para isso foi realizado o teste de MTT em linhagem celular 3T. Tests were performed in duplicates, using MEM (0 mg mL-1) as negative control. Cell viability was calculated as: absorbance average in treatments/absorbance average in controls x 100. No ensaio in vivo foram realizadas 78 feridas experimentais em coelhos que foram tratadas T. aestivum 2mg mL-1, T. aestivum 10 mg mL-1e creme não iônico por 21 dias, após foi avaliado a histologia do tricrômico de golmori e de picrosirius. Na análise estatística do ensaio de citotoxicidade foi realizado o teste de t (Graphpad), para avaliação do tricomico de golmori foi realizado o teste de fischer (Graphpad) e no picrosirius foi avaliado através de Kruskal - Wallis (Statistic 9.0). O resultado in vitro demonstrou que a dose de 2mg mL-1 foi citotóxica para as células e que doses maiores a essa apresentavam viabilidade celular. No entanto no estudo in vivo foi constatado que as feridas tratadas com essa dose apresentaram a formação de colágeno mais uniforme que as tratadas com 10 mg mL-1. Concluí-se que a dose de 2mg mL-1 do extrato aquoso de T. aestivum é eficiente no ensaio in vivo com as feridas experimentais, o que não foi observado no estudo in vitro.