Suchergebnisse

12 pags., 6 figs., 3 tabs. -- OCIS codes: (110.4500) Optical coherence tomography, (110.6880) Three-dimensional image acquisition, (100.2960) Image analysis, (330.7327) Visual optics, ophthalmic instrumentation, (330.7324) Visual optics, comparative animal models, (330.5370) Physiological optics ; Custom Spectral Optical Coherence Tomography (SOCT) provided with automatic quantification and distortion correction algorithms was used to measure the 3-D morphology in guinea pig eyes (n = 8, 30 days; n = 5, 40 days). Animals were measured awake in vivo under cyclopegia. Measurements showed low intraocular variability (<4% in corneal and anterior lens radii and <8% in the posterior lens radii, <1% interocular distances). The repeatability of the surface elevation was less than 2 µm. Surface astigmatism was the individual dominant term in all surfaces. Higher-order RMS surface elevation was largest in the posterior lens. Individual surface elevation Zernike terms correlated significantly across corneal and anterior lens surfaces. Higher-order-aberrations (except spherical aberration) were comparable with those predicted by OCT-based eye models. ; European Research Council under the European Union´s Seventh Framework Program (FP/2007-2013;) / ERC Grant Agreement [ERC-2011-AdC-294099], and Spanish Government grant FIS2011-25637; and FIS2014-56643-R to SM. ; Peer Reviewed

8 pags., 6 figs. ; Purpose: The crystalline lens undergoes morphological and functional changes with age and may also play a role in eye emmetropisation. Both the geometry and the gradient index of refraction (GRIN) distribution contribute to the lens optical properties. We studied the lens GRIN in the guinea pig, a common animal model to study myopia. Methods: Lenses were extracted from guinea pigs (Cavia porcellus) at 18 days of age (n = 4, three monolaterally treated with negative lenses and one untreated) and 39 days of age (n = 4, all untreated). Treated eyes were myopic (−2.07 D on average) and untreated eyes hyperopic (+3.3 D), as revealed using streak retinoscopy in the live and cyclopeged animals. A custom 3D spectral domain optical coherence tomography (OCT) system (λ = 840 nm, Δλ = 50 nm) was used to image the enucleated crystalline lens at two orientations. Custom algorithms were used to estimate the lens shape and GRIN was modelled with four variables that were reconstructed using the OCT data and a minimisation algorithm. Ray tracing was used to calculate the optical power and spherical aberration assuming a homogeneous refractive index or the estimated GRIN. Results: Guinea pig lenses exhibited nearly parabolic GRIN profiles. When comparing the two age groups (18- and 39 day-old) there was a significant increase in the central thickness (from 3.61 to 3.74 mm), and in the refractive index of the surface (from 1.362 to 1.366) and the nucleus (from 1.443 to 1.454). The presence of GRIN shifted the spherical aberration (−4.1 µm on average) of the lens towards negative values. Conclusions: The guinea pig lens exhibits a GRIN profile with surface and nucleus refractive indices that increase slightly during the first days of life. GRIN plays a major role in the lens optical properties and should be incorporated into computational guinea pig eye models to study emmetropisation, myopia development and ageing. ; This work was supported by European Research Council (ERC) Grant Agreement ERC-2011-AdC- 294099; ERC under the European Union's Horizon 2020 research and innovation programme (H2020-MSCA COFUND-2015 FP-713694, MULTIPLY) and under grant agreement No 779960, IMCUSTOMEYE and No 675137 (MyFUun MSCA ITN); Spanish Government Grant FIS2017-84753-R; Hunter Medical Research Institute G1400967 and the University of Newcastle (FVG 1031537 and SSP).

ARVO Annual Meeting Abstract (2019) ; Support European Research Council ERC-2011-AdG 294099; Spanish Government FIS2014-56643-R, FIS2017-84753-R; H-2020-MSCA-COFUND-2015 713694 ; Peer reviewed