Characterization of homologous and heterologous adaptive immune responses in porcine reproductive and respiratory syndrome virus infection
Altres ajuts: This work has been funded by projects AGL2008-05708-C02-02/GAN (Spanish Ministry of Education and Science) and project 245141 (PoRRSCon) of the 7th Framework Program of the European Union. Ivan Díaz has been funded by project PORCIVIR CDS-0007 of program CONSOLIDER 2010 (Spanish Ministry of Education and Science). Mariona Gimeno is funded by the fellowship program of the Universitat Autònoma de Barcelona. Liudmila Kuzemtseva is funded by the Departament d'Economia i Coneixement of Generalitat de Catalunya. Funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Our grateful thanks to Dr Jesús Hernández of CIAD AC (Hermosillo, Mexico) for critical suggestions and advice. We would like to thank also the personnel of BSL-3 facilities of CReSA for assistance. ; The present study characterized the homologous and heterologous immune response in type-I porcine reproductive and respiratory syndrome virus (PRRSV) infection. Two experiments were conducted: in experiment 1, eight pigs were inoculated with PRRSV strain 3262 and 84 days post-inoculation (dpi) they were challenged with either strain 3262 or strain 3267 and followed for the next 14 days (98 dpi). In experiment 2, eight pigs were inoculated with strain 3267 and challenged at 84 dpi as above. Clinical course, viremia, humoral response (neutralizing and non-neutralizing antibodies, NA) and virus-specific IFN-γ responses (ELISPOT) were evaluated all throughout the study. Serum levels of IL-1, IL-6, IL-8, TNF-α and TGF-β were determined (ELISA) after the second challenge. In experiment 1 primo-inoculation with strain 3262 induced viremia of ≤ 28 days, low titres of homologous NA but strong IFN-γ responses. In contrast, strain 3267 induced longer viremias (up to 56 days), higher NA titres (≤ 6 log2) and lower IFN-γ responses. Inoculation with 3267 produced higher serum IL-8 levels. After the re-challenge at 84 dpi, pigs in experiment 1 developed mostly a one week viremia regardless of ...