Suchergebnisse

Prologue -- 1 Collection of Gametes in Laboratory Animals and Preparation of Sperm for in Vitro Fertilization -- Collection of Ova for in Vitro Fertilization -- Methods Presently Used for Sperm Collection -- Differences in Sperm as a Function of Collection Method -- Preparation of Ejaculated Sperm for Use in in Vitro Fertilization -- Methods Used to Induce Capacitation of Ejaculated Sperm -- References -- 2 Ovum Collection and Induced Luteal Dysfunction in Primates -- Ovum Collection -- Evaluation of Corpus Luteum function -- Induced Folliculogenesis -- Discussion and Conclusions -- References -- 3 Analysis of Culture Media for in Vitro Fertilization and Criteria for Success -- Culture Media -- Criteria for Success -- Conclusions -- References -- 4 In Vitro Culture of the Zygote and Embryo -- General Observations on Fertilization and Early Development in Vitro -- Metabolic Requirements for Embryonic Development in Vitro -- Complex Media and Biological Fluids for Embryo Culture -- Conclusions -- References -- 5 Mechanisms of Fertilization in Mammals -- Epididymal Maturation and Capacitation of Spermatozoa -- Acrosome and Acrosome Reaction -- Hyperactivation of Spermatozoa -- Interaction of Spermatozoa with the Cumulus Oophorus -- Interaction of Spermatozoa with the Zona Pellucida -- Sperm-Egg Fusion -- Decondensation of the Sperm Nucleus and Development of Sperm and Egg Pronuclei -- Conclusions -- References -- 6 The Mammalian Egg's Block to Polyspermy -- Zona Reaction -- Zona Reaction Mechanisms -- Egg Plasma Membrane Block -- Block to Polyspermy Mechanisms -- Conclusions -- References -- 7 Gamete Interaction in the Sea Urchin A Model for Understanding the Molecular Details of Animal Fertilization -- Fertilization in the Sea Urchin -- The Plasma Membrane of Sea Urchin Sperm -- Sperm-Specific Surface Antigenicity Common to Seven Animal Phyla -- Isolation of Acrosome Granules and Identification of Bindin as the Major Component Involved in Sperm Adhesion -- Identification of a Bindin Receptor Glycoprotein from the Egg Vitelline Layer -- Conclusions -- References -- 8 Awakening of the Invertebrate Egg at Fertilization -- Sperm-Egg Fusion and the Rapid Block to Polyspermy -- Insertion and Localization of Sperm Components in the Egg -- The Cortical Reaction and Extracellular Peroxidative Reactions -- Activation of Egg Metabolism -- Conclusions -- References -- 9 Chromosome Aberrations and Mammalian Reproduction -- The Newborn -- Postimplantation Embryos -- Preimplantation Embryos -- Germ Cells -- Conclusions -- References -- 10 The Effects of Chromosomal Aneuploidy on Early Development Experimental Approaches -- Products of Aneuploid Mouse Embryos -- The Consequences of Monosomy -- Identical Twin Embryos -- Conclusions -- References -- 11 Blastocyst Fluid Formation -- Na+/ K+ ATPase and Blastocyst Fluid Accumulation -- Oxygen Consumption and Active Transport -- Developmental Aspects of Solute Transport in Blastocysts -- Conclusions -- References -- 12 Water and Electrolyte Transport by Pig Chorioallantois -- Porcine Allantoic Fluid Volume and Composition -- Effect of Lactogenic Hormones on Transport Properties of the Porcine Chorioallantoic Membrane -- Effect of Bromocryptine on Allantoic Fluid Volume and Electrolyte Composition at Day 30 of Gestation -- Discussion and Conclusions -- References -- 13 Critical Review of Embryo Transfer Procedures with Cattle -- Normalcy of Superovulated Ova -- A Note on Experimental Design -- Morphological Evaluation of Embryos -- Morphological Normalcy of Superovulated Ova with Time -- In Vitro Culture of Bovine Embryos -- Stage of the Estrous Cycle to Initiate Superovulation -- Regimens for Inseminating Superovulated Cows -- Side of Transfer -- Stage of Embryonic Development and Surgical Transfer -- Donor-Recipient Estrous Cycle Synchrony -- Factors Affecting Pregnancy Rates after Nonsurgical Transfer -- Summary and Conclusions -- References -- Epilogue.