Open Access#12017
Labeling amine-modified DNA with a novel amine-reactive coumarin: An efficient alternative for synthesizing singly fluorescent-labeled FISH probes
The suitability of using a novel coumarin TFP ester for the synthesis of fluorescent FISH probes with a single fluorophore was investigated in this work. ; This work was co-financed by FCT – Fundação para a Ciência e a Tecnologia through the project "MICROTECH-ART- Microorganisms Thriving on and Endamaging Cultural Heritage -an Analytical Rapid Tool-" (PTDC/BBB-IMG/0046/2014) and by European Union, European Regional Development Fund ALENTEJO 2020 through the project "HIT3CH - HERCULES Interface for Technology Transfer and Teaming in Cultural Heritage" (ALT20-03-0246-FEDER-000004).Marina González-Pérez acknowledges FCT for the economic support through the post-doctoral grant SFRH/BPD/100754/2014.