Suchergebnisse

Semiotics / semiology

[EN] Myocardial tissue lacks the ability to regenerate itself significantly following a myocardial infarction. Thus, new strategies that could compensate this lack are of high interest. Cardiac tissue engineering (CTE) strategies are a relatively new approach that aims to compensate the tissue loss using combination of biomaterials, cells and bioactive molecules. The goal of the present study was to evaluate cell survival and growth, seeding capacity and cellular phenotype maintenance of subcutaneous adipose tissue-derived progenitor cells in a new synthetic biomaterial scaffold platform. Specifically, here we tested the effect of the RAD16-I peptide gel in microporous poly(ethyl acrylate) polymers using two-dimensional PEA films as controls. Results showed optimal cell adhesion efficiency and growth in the polymers coated with the self-assembling peptide RAD16-I. Importantly, subATDPCs seeded into microporous PEA scaffolds coated with RAD16-I maintained its phenotype and were able to migrate outwards the bioactive patch, hopefully toward the infarcted area once implanted. These data suggest that this bioimplant (scaffold/RAD16-I/cells) can be suitable for further in vivo implantation with the aim to improve the function of affected tissue after myocardial infarction. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3419-3430, 2015. ; Contract grant sponsor: European Union Seventh Framework Programme; contract grant number: 229239 ; Castells-Sala, C.; Martínez Ramos, C.; Vallés Lluch, A.; Monleón Pradas, M.; Semino, C. (2015). In vitro development of bioimplants made up of elastomeric scaffolds with peptide gel filling seeded with human subcutaneous adipose tissue-derived progenitor cells. Journal of Biomedical Materials Research Part A. 103(11):3419-3430. https://doi.org/10.1002/jbm.a.35482 ; S ; 3419 ; 3430 ; 103 ; 11 ; Persidis, A. (1999). Tissue engineering. Nature Biotechnology, 17(5), 508-510. doi:10.1038/8700 ; Venugopal, J. R., Prabhakaran, M. P., Mukherjee, S., Ravichandran, R., Dan, ...

[EN] Tissue phantoms are widely used for assessing the interaction between the electromagnetic waves and the human body. These are especially key in body area networks, where the body itself acts as the propagation medium since transmission is highly influenced by its diverse dielectric properties. Gels are suitable materials because of their high water content, which is required to mimic the dielectric properties of most tissues. In this paper, PHEA gels are suggested for achieving those properties due to their synthetic nature, which gives them the possibility to be swollen reversibly in more types of mixtures, in addition to water. These gels can be tailored to control the amount of liquid they embed so that they can imitate different body tissues in a wide bandwidth (2¿26.5 GHz), which includes most of the current mobile communication and medical bands. This versatility offers the chance to create heterogeneous models of particular regions of the body, and thus improve the test realism. In addition, they own better mechanical and stability properties than the widely used agar or gelatin. ; This work was supported in part by the Universitat Politecnica de Valencia-Institut d'Investigacio Sanitaria La Fe (UPV-IIS La Fe) Program [Early Stage Colon Tumour Diagnosis by Electromagnetic Reflection (STuDER), 2016 and Electromagnetic Probe for Early Tumour Detection (EMOTE), 2017], in part by the Universitat Politecnica de Valencia through the Programa de Ayudas de Investigacion y Desarrollo under Grant PAID-01-16, and in part by the European Union's H2020: MSCA: ITN Program for the "mmWave Communications in the Built Environments - WaveComBE" Project under Grant 766231. ; Castelló-Palacios, S.; Garcia-Pardo, C.; Alloza-Pascual, M.; Fornés Leal, A.; Cardona Marcet, N.; Vallés Lluch, A. (2019). Gel Phantoms for Body Microwave Propagation in the (2 to 26.5) GHz Frequency Band. IEEE Transactions on Antennas and Propagation. 67(10):6564-6573. https://doi.org/10.1109/TAP.2019.2920293 ; S ; 6564 ; 6573 ; 67 ; 10

[EN] An in-body sensor network is that in which at least one of the sensors is located inside the human body. Such wireless in-body sensors are used mainly in medical applications, collecting and monitoring important parameters for health and disease treatment. IEEE Standard 802.15.6-2012 for wireless body area networks (WBANs) considers in-body communications in the Medical Implant Communications Service (MICS) band. Nevertheless, high-data-rate communications are not feasible at the MICS band because of its narrow occupied bandwidth. In this framework, ultrawideband (UWB) systems have emerged as a potential solution for in-body highdata-rate communications because of their miniaturization capabilities and low power consumption. ; This work was supported by the Programa de Ayudas de Investigación y Desarrollo (PAID-01-16) at the Universitat Politècnica de València, Spain; by the Ministerio de Economía y Competitividad, Spain (TEC2014-60258-C2-1-R); and by the European FEDER funds. It was also funded by the European Union's H2020:MSCA:ITN program for the Wireless In-Body Environ-ment Communication–WiBEC project under grant 675353. ; Garcia-Pardo, C.; Andreu-Estellés, C.; Fornés Leal, A.; Castelló-Palacios, S.; Pérez-Simbor, S.; Barbi, M.; Vallés Lluch, A. (2018). Ultrawideband Technology for Medical In-Body Sensor Networks: An Overview of the Human Body as a Propagation Medium, Phantoms, and Approaches for Propagation Analysis. IEEE Antennas and Propagation Magazine. 60(3):19-33. https://doi.org/10.1109/MAP.2018.2818458 ; S ; 19 ; 33 ; 60 ; 3

[EN] Contractile restoration of myocardial scars remains a challenge with important clinical implications. Here, a combination of porous elastomeric membrane, peptide hydrogel, and subcutaneous adipose tissue-derived progenitor cells (subATDPCs) was designed and evaluated as a bioimplant for cardiac regeneration in a mouse model of myocardial infarction. SubATDPCs were doubly transduced with lentiviral vectors to express bioluminescent-fluorescent reporters driven by constitutively active, cardiac tissue-specific promoters. Cells were seeded into an engineered bioimplant consisting of a scaffold (polycaprolactone methacryloyloxyethyl ester) filled with a peptide hydrogel (PuraMatrix(TM)), and transplanted to cover injured myocardium. Bioluminescence and fluorescence quantifications showed de novo and progressive increases in promoter expression in bioactive implant-treated animals. The bioactive implant was well adapted to the heart, and fully functional vessels traversed the myocardium-bioactive implant interface. Treatment translated into a detectable positive effect on cardiac function, as revealed by echocardiography. Thus, this novel implant is a promising construct for supporting myocardial regeneration. ; The research leading to these results received funding from the European Union Seventh Framework Programme (Project RECATABI, 7FP/2007-2013) under grant agreement number 229239. This work was also supported by Ministerio de Ciencia e Innovación (SAF2011- 30067-C02-01), Fundació La Marató de TV3 (080330), Red de Terapia Celular-TerCel (RD12/0019/0029), Red Cardio-vascular (RD12/0042/0047), Sociedad Española de Cardiología, and Fundació Privada Daniel Bravo Andreu ; Soler-Botija, C.; Bago, JR.; Llucia-Valldeperas, A.; Vallés Lluch, A.; Castells-Sala, C.; Martinez-Ramos, C.; Fernandez-Muinos, T. (2014). Engineered 3D bioimplants using elastomeric scaffold, self-assembling peptide hydrogel, and adipose tissue-derived progenitor cells for cardiac regeneration. American Journal of Translational Research. ...

[EN] Ischemia produced as a result of myocardial infarction might cause moderate or severe tissue death. Studies under development propose grafting stem cells into the affected area and we hypothesize that this mechanism could be enhanced by the application of a "bioactive implant." The implant herein proposed consists of a thin porous elastomeric membrane, filled with self-assembling nanofibers and human subcutaneous adipose tissue derived progenitor cells. We describe the development and characterization of two elastomeric membranes: poly(ethyl acrylate) (PEA) and poly(caprolactone 2-(methacryloyloxy) ethyl ester) (PCLMA). Both are a good material support to deliver cells within a soft self-assembling peptide and are elastic enough to withstand the stresses arising from the heartbeat. Both developed composites (PEA and PCLMA, combined with self-assembling peptide) equally facilitate the propagation of electrical pulses and maintain their genetic profile of the seeded cells. Preliminary studies with small animal models suggest that, at short times, the bioimplant shows good adhesion with the myocardium. After three days cells loaded in the patch remain alive at the implanted site. We propose that the bioactive patch (elastomeric membranes with self-assembling peptide and cells) could increase the efficacy of future cardiac cell therapy by improving cell immobilization and survival at the affected site. ; The authors wish to thank the Department of Cardiac Surgery (Hospital Germans Trias i Pujol, Badalona) for their collaboration in obtaining human samples, Dr. Bago for his kind contribution in the cell transduction process and BLI analysis, and Joan Gilabert from Biomaterials Laboratory (GEMAT, IQS-School of Engineering) who kindly helped them with wettability measurements. The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under Grant agreement no. 229239. This work was also supported by Grants from Ministerio de Educacion y Ciencia ...

Cumulative toxicity from weekly paclitaxel (myalgia, peripheral neuropathy, fatigue) compromises long-term administration. Preclinical data suggest that the burden of critically short telomeres ( 21.9% CSTs) had 2-fold higher number of neuropathy (P = 0.04) or fatigue (P = 0.019) episodes and >3-fold higher number of myalgia episodes (P = 0.005). The average telomere length was unrelated to the incidence of side effects.The percentage of CSTs, but not the average telomere size, is associated with weekly paclitaxel-derived toxicity. ; This work was supported by the Fondo de Investigación Sanitaria [FIS PI10/00288 and FIS PI13/00430]; AECC Scientific Foundation [Beca de Retorno-2010, to MQF]; Spanish Ministry of Economy and Competitiveness Projects [SAF2013-45111-R]; Madrid Regional Government Projects [S2010/BMD- 2303]; AXA ...