Suchergebnisse

Puberty, governed by the endocrine system, marks the onset of reproductive functions in animals and humans through a series of physiological and biological transformations. Although the mycotoxin DON can disrupt hormonal balance and cause reproductive abnormalities, its impact on puberty-associated reproductive changes remains understudied. Considering the increased exposure of children and adolescents to DON, our study aimed to elucidate its influence on follicular integrity and the expression of pro-apoptotic proteins (BAX and Caspase-3) and anti-apoptotic protein (BCL-2) in juvenile rat ovarian tissue. We divided ten 28-day-old prepubertal Wistar rats into two dietary groups for 28 days: a control group with a mycotoxin-free diet and a DON group with a diet containing 10 mg DON/Kg. After the experiment, ovaries and uterus weights were recorded, and the ovaries underwent morphometric and immunohistochemical analysis. DON exposure led to significant reductions in both ovarian and uterine weights. Although DON intake did not change the number of ovarian follicles across developmental stages, we observed an increased expression of BAX and Caspase-3 and a decreased BCL-2 expression in most follicular stages and corpora lutea. In summary, DON exposure during puberty can interfere with apoptotic processes in diverse ovarian cell populations during early adulthood.