Suchergebnisse

Lymph node stromal cells (LNSCs) closely regulate immunity and self-tolerance, yet key aspects of their biology remain poorly elucidated. Here, comparative transcriptomic analyses of mouse LNSC subsets demonstrated the expression of important immune mediators, growth factors and previously unknown structural components. Pairwise analyses of ligands and cognate receptors across hematopoietic and stromal subsets suggested a complex web of crosstalk. Fibroblastic reticular cells (FRCs) showed enrichment for higher expression of genes relevant to cytokine signaling, relative to their expression in skin and thymic fibroblasts. LNSCs from inflamed lymph nodes upregulated expression of genes encoding chemokines and molecules involved in the acute-phase response and the antigen-processing and antigen-presentation machinery. Poorly studied podoplanin (gp38)-negative CD31− LNSCs showed similarities to FRCs but lacked expression of interleukin 7 (IL-7) and were identified as myofibroblastic pericytes that expressed integrin α7. Together our data comprehensively describe the transcriptional characteristics of LNSC subsets. ; National Institutes of Health (U.S.) (grant R01 DK074500) ; National Institutes of Health (U.S.) (grant P01 AI045757) ; National Institutes of Health (U.S.) (grant R24 AI072073) ; National Institutes of Health (U.S.) (grant R01 AI063428-06) ; National Institutes of Health (U.S.) (grant R01 DE019917) ; National Institutes of Health (U.S.) (grant GM38903) ; Dana-Farber Cancer Institute ; Seventh Framework Programme of the European Union (Marie Curie International Outgoing Fellowship 220044)