Intensive poultry farming creates the ideal environment for pathogen concentration and transmission. The presence of thousands of birds in an enclosed, warm, and dusty atmosphere is ideal for the transmission of infectious diseases from birds to humans. This study was conducted to assess the indoor and outdoor air quality of different poultry types in Edo State, Nigeria. The physicochemical conditions of the air around the poultry environments differed with location and poultry types. The concentrations of carbon dioxide (CO2,), nitrous oxide (N2O), hydrogen sulphide (H2S) as well as particulate matter (PM10) were all within recommended limits established by the World Health Organization. However, significant elevations in Ammonia (NH3) and sulphur dioxide (SO2) levels were observed in substandard poultry farms across the locations. Total bacterial counts ranged from 1.38CFU/m5 – 90.35 x105CFU/m3 irrespective of location and poultry type. Within the poultry types, bacteria count inside the poultry environment (3.11 x105CFU/m3) significantly differed from concentrations outside the poultry environment (22.58 x105CFU/m3, p<0.05). The Lowest microbial counts were obtained in the standard poultry farms. Molecular identifications revealed the presence of Escherichia coli, Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Bacillus subtilis as the bacterial isolates whereas Fusarium oxysporum, Aspergillus niger, Rhizopus stolonifer, Trichoderma polysporum, Aspergillus fumigatus were the fungal isolates. Staphylococcusaureus was the most predominant bacterial species (25%) while Aspergillus niger was the most predominant fungal species (30%).
"Water is an essential resource and its purity should not be negotiated when it comes to human consumption. This study investigated the antibacterial activity of Garcinia kola and Hunteria umbellata seed and epicarp on some bacterial isolates from sachet water. Duplicate samples of ten brands of sachet water were purchased from sales outlets around Ugbowo community, Benin City. Plate count techniques, minimum inhibitory and minimum bactericidal concentrations of the extracts were adopted in this investigation. Heterotrophic bacterial counts revealed highest range of 3.72±0.50x102 cfu/mL in EJ water and lowest in IB water (0.00±0.0x102 cfu/mL) while total coliform counts revealed its highest value in EJ water (3.62±0.30x102 cfu/mL) and lowest value (0.00±0.0 x102 cfu/mL), in OL, IB, NOS and UNI water. Aeromonas sp., Bacillus sp., Pseudomonas sp., Staphylococcus aereus and Enterobacter sp. were isolated. The antimicrobial susceptibility profile revealed varying zones of inhibition of 4 mm for Aeromonas sp., for Garcinia Kola and 20 mm recorded against Staphylococcus aureus and Bacillus sp. for Hunteria umbellata epicarp extract. The Minimum Inhibitory Concentration of susceptible bacteria to Hunteria umbellata and Garcinia kola extract were 6.25mg/mL and 12.5mg/mL, respectively, except Aeromonas sp. which had 50 mg/mL. Hunteria umbellata epicarp had a greater bactericidal effect of 6.25mg/mL against Staphylococcus aureus, while Garcinia kola had its greatest bactericidal effect on Enterobacter sp. with a minimum bactericidal concentration of 12.5mg/mL. This study has revealed the potentials of Hunteria umbellata epicarp and Garcinia kola as effective natural therapeutic agents against some harmful bacteria, preventing their pathogenic effect. Keywords: Herbal extracts, Hygiene education, Minimum Bactericidal Concentration, Minimum Inhibitory Concentration, Sachet water."