Suchergebnisse

Taxol, a natural product derived from Taxus, is one of the most effective natural anticancer drugs and the biosynthetic pathway of Taxol is the basis of heterologous bio-production. Here, we report a high-quality genome assembly and annotation of Taxus yunnanensis based on 10.7 Gb sequences assembled into 12 chromosomes with contig N50 and scaffold N50 of 2.89 Mb and 966.80 Mb, respectively. Phylogenomic analyses show that T. yunnanensis is most closely related to Sequoiadendron giganteum among the sampled taxa, with an estimated divergence time of 133.4−213.0 MYA. As with most gymnosperms, and unlike most angiosperms, there is no evidence of a recent whole-genome duplication in T. yunnanensis. Repetitive sequences, especially long terminal repeat retrotransposons, are prevalent in the T. yunnanensis genome, contributing to its large genome size. We further integrated genomic and transcriptomic data to unveil clusters of genes involved in Taxol synthesis, located on the chromosome 12, while gene families encoding hydroxylase in the Taxol pathway exhibited significant expansion. Our study contributes to the further elucidation of gymnosperm relationships and the Taxol biosynthetic pathway. ; The National Key R&D Program of China from the Ministry of Science and Technology of China, the National Natural Science Foundation of China and Karst Science Research Center of Guizhou Province; Scientific and technological innovation project of China Academy of Chinese Medical Sciences; National Key Research and Development Project and National Natural Science Foundation of China; the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program and Ghent University. ; https://www.nature.com/commsbio ; am2022 ; Biochemistry ; Genetics ; Microbiology and Plant Pathology