Adaptation of hydroxymethylbutenyl diphosphate reductase enables volatile isoprenoid production
Volatile isoprenoids produced by plants are emitted in vast quantities into the atmosphere, with substantial effects on global carbon cycling. Yet, the molecular mechanisms regulating the balance between volatile and non-volatile isoprenoid production remain unknown. Isoprenoids are synthesised via sequential condensation of isopentenyl pyrophosphate (IPP) to dimethylallyl pyrophosphate (DMAPP), with volatile isoprenoids containing fewer isopentenyl subunits. The DMAPP:IPP ratio could affect the balance between volatile and non-volatile isoprenoids, but the plastidic DMAPP:IPP ratio is generally believed to be similar across different species. Here we demonstrate that the ratio of DMAPP:IPP produced by hydroxymethylbutenyl diphosphate reductase (HDR/IspH), the final step of the plastidic isoprenoid production pathway, is not fixed. Instead, this ratio varies greatly across HDRs from phylogenetically distinct plants, correlating with isoprenoid production patterns. Our findings suggest that adaptation of HDR plays a previously unrecognised role in determining in vivo carbon availability for isoprenoid emissions, directly shaping global biosphere-atmosphere interactions. ; Research at the University of Queensland was funded by an Australian Research Council grant to LKN and CEV. Research at the Center for Biosustainability was supported by The Novo Nordisk Foundation under NFF grant number NNF10CC1016517. JPG was supported by a Marie Curie International outgoing Fellowship within the 7th European Community Framework Programme. CEV was supported by Queensland Government Smart Futures and Accelerate Fellowships. Metabolomics Australia is part of the Bioplatforms Australia network, funded through the Australian Government's National Collaborative Research Infrastructure Strategy (NCRIS). ; Peer reviewed