Deciphering the modulation of enniatins biosynthesis by Fusarium avenaceum under the effect of abiotic stress ; Décrypter la modulation de la biosynthèse d'enniatines par Fusarium avenaceum sous l'effet de stress abiotiques
Fusarium avenaceum is a filamentous fungus infecting many plants and is one of the causal agents of the Fusarium Head Blight disease in wheat and barley. During plant infection, the fungus can produce secondary metabolites that are toxic for human and livestock (mycotoxins), among which the enniatins. Enniatins belong to the group of "emerging mycotoxins": first identified as early as 1950, the increasing occurrence of enniatins has only been documented over the last twenty years. Currently, enniatins are neither routinely analysed in grain harvests nor legislatively regulated, even though they are widespread and can be encountered in high concentrations. Diversity of enniatin-producing strains as well as the mechanisms by which their biosynthesis can be modulated during plant infection and/or in response to environmental conditions are poorly known. These gaps of knowledge hinder the development of tools for controlling and mitigating their presence in crops. In the present study, a comprehensive characterisation of the phenotypic diversity within the F. avenaceum species has been carried out. One of the studied phenotypic traits was the effect of pH variations on the fungal growth and toxin production, which has led to evidence various response profiles between strains. To go further, the role of FavPac1, the homologue of pacC/RIM101 encoding a transcriptional factor involved in the response to ambient pH in fungi, has been studied. Construction and phenotyping of deleted mutants, Fav∆Pac1, for four different F. avenaceum isolates have allowed suggesting an involvement of FavPac1 in the modulation of enniatin production and of the expression of biosynthetic genes induced by pH variations. Lastly, with the aim to identify alternatives to synthetic fungicides, the capacity of plant phenolic compounds to inhibit the yield of enniatins has been investigated. The antifungal and anti-mycotoxin properties of hydroxycinnamic acids have been shown; ferulic acid has been highlighted as the most potent compound. ...