In Obese Patients With Type 2 Diabetes, Mast Cells in Omental Adipose Tissue Decrease the Surface Expression of CD45, CD117, CD203c, and FcϵRI
This work was supported by Instituto de Salud Carlos III (grants PI15/01361 and PI18/01947), and Conserjeria de Salud y Familias, Junta de Andalucia (grant PIN-0235-2019). Instituto de Salud Carlos III (ISCIII) is a public organization that belongs to the Spanish Ministry of Health. ISCIII funds scientific projects on competitive open calls. Conserjeria de Salud y Familias, Junta de Andalucia is a department of the regional government of Andalucia (Spain) that funds scientific projects on competitive open calls. ; The paradigm of mast cells in type 2 diabetes is changing. Although they were first considered deleterious inflammatory cells, now they seem to be important players driving adipose tissue homeostasis. Here we have employed a flow cytometry-based approach for measuring the surface expression of 4 proteins (CD45, CD117, CD203c, and FcϵRI) on mast cells of omental (o-WAT) and subcutaneous white adipose tissue (s-WAT) in a cohort of 96 patients with morbid obesity. The cohort was split into three groups: non-T2D, pre- T2D, and T2D. Noteworthy, patients with T2D have a mild condition (HbA1c <7%). In o- WAT, mast cells of patients with T2D have a decrease in the surface expression of CD45 (p=0.0013), CD117 (p=0.0066), CD203c (p=0.0025), and FcϵRI (p=0.043). Besides, in s- WAT, the decrease was seen only in CD117 (p=0.046). These results indicate that T2D affects more to mast cells in o-WAT than in s-WAT. The decrease in these four proteins has serious effects on mast cell function. CD117 is critical for mast cell survival, while CD45 and FcϵRI are important for mast cell activation. Additionally, CD203c is only present on the cell surface after granule release. Taking together these observations, we suggest that mast cells in o-WAT of patients with T2D have a decreased survival, activation capacity, and secretory function. ; Instituto de Salud Carlos III ; European Commission PI15/01361 PI18/01947 ; Junta de Andalucia FPU18/04432 PIN-0235-2019 ; Spanish Ministry of Health. ISCIII funds ; Junta de ...