Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery
12 páginas, 7 figuras, 1 tabla. Material suplementario en: https://doi.org/10.1016/S0092-8674(03)01025-0. The SUS1 sequences have been deposited in GenBank with the accession number AY278445. ; Gene expression is a coordinated multistep process that begins with transcription and RNA processing in the nucleus followed by mRNA export to the cytoplasm for translation. Here we report the identification of a protein, Sus1, which functions in both transcription and mRNA export. Sus1 is a nuclear protein with a concentration at the nuclear pores. Biochemical analyses show that Sus1 interacts with SAGA, a large intranuclear histone acetylase complex involved in transcription initiation, and with the Sac3-Thp1 complex, which functions in mRNA export with specific nuclear pore proteins at the nuclear basket. DNA macroarray analysis revealed that Sus1 is required for transcription regulation. Moreover, chromatin immunoprecipitation showed that Sus1 is associated with the promoter of a SAGA-dependent gene during transcription activation. Finally, mRNA export is impaired in sus1 mutants. These data provide an unexpected connection between the SAGA histone acetylase complex and the mRNA export machinery ; This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 352, Leibniz-Programm) and Fonds der Chemischen Industrie, R.R. by a grant from NIH, and J.E.P.-O. was supported by (GEN2001-4707-C08-07) from Ministerio de Ciencia y Tecnologı́a and (QLRI-CT-1999-01333) from the European Union. S.R.-N. is a holder of a Marie Curie fellowship. ; Peer reviewed