Suchergebnisse

In many plant species, an exposure to a sublethal temperature triggers an adaptative response called acclimation. This response involves an extensive molecular reprogramming that allows the plant to further survive to an otherwise lethal increase of temperature. A related response is also launched under an abrupt and lethal heat stress that, in this case, is unable to successfully promote thermotolerance and therefore ends up in plant death. Although these molecular programmes are expected to have common players, the overlapping degree and the specific regulators of each process are currently unknown. We have carried out a high-throughput comparative proteomics analysis during acclimation and during the early stages of the plant response to a severe heat stress that lead Arabidopsis seedlings either to survival or death. This analysis dissects these responses, unravels the common players and identifies the specific proteins associated with these different fates. Thermotolerance assays of mutants in genes with an uncharacterized role in heat stress demonstrate the relevance of this study to uncover both positive and negative heat regulators and pinpoint a pivotal role of JR1 and BAG6 in heat tolerance. ; The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement 260468 to M. Mar Castellano and from the grants RTA2013-00027-00-00 and S2013/ABI-2734-CM. We thank Dr. A. Munoz for critical reading of the manuscript, Dr. R. Toribio for his help in the annotation of gene descriptions and Dr. Hara-Nishimura and Dr. Hans-Peter Mock for kindly providing the OLEO2 and HSP90.1 antibodies, respectively. Plant Response is also thanked for its support in the project. The authors declare no conflict of interest. ; Sí