Suchergebnisse

El propósito del presente trabajo es identificar los problemas de salud que se presenta en la población que está dentro del proceso del fenómeno migratorio México-Estados Unidos, aquellos que migran de manera estacional o permanente y los que participan quedándose en la comunidad de origen, desde cada uno de los momentos por los que atraviesan, de la partida, durante el traslado y a la llegada, así como los riesgos a lo que se enfrentan, las causas y las principales enfermedades que padecen. Con la intención de brindar un panorama acerca de las necesidades que experimentan los migrantes y con esto, se puedan diseñar estrategias orientadas a disminuir las problemáticas de salud, al desarrollo de programas de intervención que favorezcan la salud y estrategias para prevenir la enfermedad.

Este artículo presenta una reflexión en torno a la construcción de ciudadanía, a partir de un diálogo entre la filosofía y la literatura, con el fin de encontrar ese entre nos que las relaciona y los modos en que afectan a los sujetos. En particular, el documento se centra en la comprensión del mal político, a través de la posibilidad de situarlo. Este ejercicio permite que se haga un juicio sobre las acciones que acometen los hombres y los marcos de interpretación que configura el poder para dominarlos. Una vez se reconoce este dominio, se da paso a la idea del hombre compasional, como aquel que puede ejercer un tipo de ciudadanía diferente, que ataca la idea de servidumbre voluntaria y la esclavitud que se promueven. La intención central está acompañada por la caracterización de las acciones del hombre y su posibilidad de ser un perturbador, capaz de mantener la política y la democracia en continuo movimiento

Semaphorin 3A (Sema3A) is a cell-secreted protein that participates in the axonal guidance pathways. Sema3A acts as a canonical repulsive axon guidance molecule, inhibiting CNS regenerative axonal growth and propagation. Therefore, interfering with Sema3A signaling is proposed as a therapeutic target for achieving functional recovery after CNS injuries. It has been shown that Sema3A adheres to the proteoglycan component of the extracellular matrix (ECM) and selectively binds to heparin and chondroitin sulfate-E (CS-E) glycosaminoglycans (GAGs). We hypothesize that the biologically relevant interaction between Sema3A and GAGs takes place at Sema3A C-terminal polybasic region (SCT). The aims of this study were to characterize the interaction of the whole Sema3A C-terminal polybasic region (Sema3A 725–771) with GAGs and to investigate the disruption of this interaction by small molecules. Recombinant Sema3A basic domain was produced and we used a combination of biophysical techniques (NMR, SPR, and heparin affinity chromatography) to gain insight into the interaction of the Sema3A C-terminal domain with GAGs. The results demonstrate that SCT is an intrinsically disordered region, which confirms that SCT binds to GAGs and helps to identify the specific residues involved in the interaction. NMR studies, supported by molecular dynamics simulations, show that a new peptoid molecule (CSIC02) may disrupt the interaction between SCT and heparin. Our structural study paves the way toward the design of new molecules targeting these protein–GAG interactions with potential therapeutic applications. ; This work was funded by the European Union Seventh Framework Programme (FP7/2007–2013) under Project VISION, grant No. 304884, the Spanish Ministry of Science and Innovation/Spanish Research Agency (MCI/AEI/FEDER, RTI2018–096182-B-I00) and AGAUR (2017 SGR 208). ; Peer reviewed

Objectives To explore the macrophage profiles in symptomatic and asymptomatic forms of AP through phenotypic and functional analyses. Material and methods Cross-sectional study. Apical tissue/lesion samples were collected from patients with clinical diagnosis of AAP (n = 51) or SAP (n = 45) and healthy periodontal ligament (HPL) from healthy patients as controls (n = 14), all with indication of tooth extraction. Samples were digested, cells were stained for CD14, M1 (CD64, CD80), and M2 (CD163, CD206) phenotypic surface markers and analyzed by flow cytometry. Functional cytokine profiles L-6, IL-12, TNF-alpha, IL-23 (M1), IL-10, and TGF-beta (M2) were determined by qPCR. Results Higher macrophage M1/M2 ratio (CD64(+)CD80(+)/CD163(+)CD206(+)) along with lower CD163 mean fluorescence intensity (MFI) were found in SAP compared to AAP and controls (p < 0.05). IL-6, IL-12, TNF-alpha, IL-23 (M1), and IL-10 mRNA (M2) were upregulated, whereas TGF-beta mRNA (M2) was downregulated in apical lesions compared to controls. Specifically, IL-6 and IL-23 (M1) were upregulated in SAP compared with AAP and controls (p < 0.05). The data were analyzed with Kruskal-Wallis test. Conclusions Macrophages exhibited a polarization switch towards M1 in AL. SAP exhibited a reduced M2 differentiation profile based on a reduction of CD163 expression levels in SAP over AAP. Specifically, IL-6 and IL-23 were augmented SAP over AAP, suggesting a role in the severity of apical lesions. ; Chilean Government CONICYT 21171640