Mapping wheat rust based on high spatial resolution satellite imagery
In: Computers and Electronics in Agriculture, Band 152, S. 109-116
7 Ergebnisse
Sortierung:
In: Computers and Electronics in Agriculture, Band 152, S. 109-116
In: Computers and electronics in agriculture: COMPAG online ; an international journal, Band 223, S. 109089
ISSN: 1872-7107
In: Environmental science and pollution research: ESPR, Band 22, Heft 7, S. 4932-4941
ISSN: 1614-7499
In: Waste management: international journal of integrated waste management, science and technology, Band 96, S. 47-56
ISSN: 1879-2456
In: Ecotoxicology and environmental safety: EES ; official journal of the International Society of Ecotoxicology and Environmental safety, Band 232, S. 113273
ISSN: 1090-2414
In antibiotics, β-lactam is one kind of major concern acknowledged as an unavoidable contaminant in milk. Thus, a facile and sensitive method is essential for rapid β-lactam antibiotics detection. In our work, a specific electrochemical receptor sensor based on the graphene/thionine (GO/TH) composite was established. The mechanism of the electrochemical receptor sensor was a direct competitive inhibition of the binding of horseradish peroxidase-labeled ampicillin (HRP-AMP) to the mutant BlaR-CTD protein by free β-lactam antibiotics. Then, horseradish peroxidase (HRP) catalyzed the hydrolysis of the substrate hydrogen peroxide (H(2)O(2)), which produced an electrochemical signal. Under optimal experimental conditions, this method could quantitatively detect cefquinome from 0.1 to 8 μg L(−1) and with the limit of detection (LOD) of 0.16 μg L(−1), much lower than the maximum residue limit (MRL) of 5 μg L(−1) set by the European Union. In addition, the LOD of spiked milk samples with cefalexin, cefquinoxime, cefotafur, penicillin G and ampicillin were 14.88 μg L(−1), 2.46 μg L(−1), 17.16 μg L(−1), 0.06 μg L(−1), 0.21 μg L(−1) and the limits of quantitation (LOQ) were 36.09 μg L(−1), 5.40 μg L(−1), 41.45 μg L(−1), 0.13 μg L(−1), 0.42 μg L(−1), respectively. The sensor showed a favorable recovery of 84.89–102.44%. Moreover, the electrochemical receptor sensor was successfully applied to assay β-lactam antibiotics in milk, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).
BASE
SSRN