Suchergebnisse

Este artículo es producto de la reflexión de las autoras, desde su práctica docente en tiempos de cuarentena por covid-19, mediada por el uso de las tecnologías de la información y la comunicación. Se hace referencia a la formación integral como un punto de partida para pensar el proceso socioeducativo; la discusión sobre los vínculos y la intervención profesional; las tendencias sobre intervención en Trabajo Social virtual; los elementos para comprender el uso de las tecnologías de la información y la comunicación en la educación; y, por último, se sugieren algunos aprendizajes y desafíos, producto de la revisión de literatura y la experiencia.

Las investigaciones en Colombia sobre construcción de paz desde la perspectiva de las mujeres son escasas, en particular en la especificidad de mujeres rurales, aunque muchas de las experiencias son protagonizadas en dicho ámbito. En este artículo se hace una revisión documental de diversos estudios que han abordado el tema y que se agrupan en tres campos: 1) la discusión sobre el movimiento social de mujeres por la paz; 2) los aportes de las mujeres a la construcción de paz desde los contextos particulares y 3) la participación de las mujeres en procesos de paz. Posteriormente, se exponen los principales hallazgos teóricos, conceptuales y metodológicos. Finalmente, en las conclusiones se ratifica que no existen investigaciones que teórica y analíticamente reflexionen sobre la triada construcción de paz-mujer-ruralidad y que, por el contrario, hay una serie de desafíos que invitan a profundizar en este campo de cara a los procesos de implementación de acuerdos de paz.

Este artículo presenta los aportes teóricos y metodológicos de Evelyn H. Davison a partir del texto Trabajo social de casos. La revisión de los aportes de Davison tiene un valor y significado fundamentales, puesto que permite reflexionar sobre los avances, las continuidades y las rupturas que en el ejercicio y en la teorización del Trabajo Social se han generado con el pasar de los años, los cambios socioculturales y el surgimiento de marcos teóricos y conceptuales diferentes a los de los periodos en los que la profesión tuvo origen.

This document is the unedited Author's version of a Submitted Work that was subsequently accepted for publication in Analytical Chemistry copyright © American Chemical Society after peer review. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.7b04788 . ; European Union's Horizon 2020

The research was performed at Comuna seven of Cali, Colombia in order to understand the process of community empowerment in a particular context, respect to the relationship between the healthful territories construction and the local development, recognizing (examining closely) the characteristics of citizen participation, the interaction between the different actors, power relations, the conflicts in the planning scenario and the way of use it. It was realized a qualitative interpretative research, with ethnographic design, case method, with community and public functionaries representatives, who participated in Territorial Planning Committee. The results show that the scenarios of participative planning are strategic for public health and help the process of community empowerment because the community makes decisions that qualify their capacity of participation and organization through managing and controlling of affaires that impact the conditions of health of population. The community empowerment more than a strategy is the politic dimension of Public Health that should change its sectorial condition toward the planning of global actions in the framework of integral development. In the construction of healthful territories the participation of the communitarian actor responds to their own motivations and interests and not to the originating ones from the governmental sector. In the Committee of Territorial Planning power relations are pronounced, generating conflicts that as well are fed on corrupted political practices, which interfere and turn slow the process of community empowerment in their three constituent categories, participation, decision-making and management. ; La investigación cualitativa interpretativa, diseño etnográfico, en la modalidad de caso, se realizó en la Comuna siete de Santiago de Cali, Colombia con representantes comunitarios y funcionarios públicos que participaron en el Comité de Planificación Territorial, para comprender el proceso de empoderamiento comunitario en un contexto particular, respecto de la relación entre la construcción de territorios saludables y el desarrollo local, reconociendo las características de la participación ciudadana, las interacciones entre diferentes actores, las relaciones de poder, los conflictos en el escenario de la planificación y la forma como se utiliza este escenario. Los escenarios de planificación participativa son estratégicos para la Salud Pública y favorecen el proceso de empoderamiento, porque el actor comunitario toma de decisiones que le permiten cualificar su capacidad de participación y organización, haciendo gestión y control de los asuntos que impactan las condiciones de salud de la población. El empoderamiento comunitario más que una estrategia, es la dimensión política de la Salud Pública; debe pasar de su condición sectorial a la planificación de acciones globales en el marco del desarrollo integral. En la construcción de territorios saludables la participación del actor comunitario responde a intereses propios y no a los provenientes del sector gubernamental. En el Comité de Planificación Territorial se manifiestan relaciones de poder que generan conflictos, que a su vez obedecen a prácticas clientelistas, lo que interfiere el proceso de empoderamiento comunitario en sus tres categorías constitutivas: participación, decisión y gestión.

The RNA polymerase II-associated protein 1 (RPAP1) is conserved across metazoa and required for stem cell differentiation in plants; however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of cell identity genes and for cell viability. Depletion of RPAP1 triggers cell de-differentiation, facilitates reprogramming toward pluripotency, and impairs differentiation. Mechanistically, we show that RPAP1 is essential for the interaction between RNA polymerase II (RNA Pol II) and Mediator, as well as for the recruitment of important regulators, such as the Mediator-specific RNA Pol II factor Gdown1 and the C-terminal domain (CTD) phosphatase RPAP2. In agreement, depletion of RPAP1 diminishes the loading of total and Ser5-phosphorylated RNA Pol II on many genes, with super-enhancer-driven genes among the most significantly downregulated. We conclude that Mediator/RPAP1/RNA Pol II is an ancient module, conserved from plants to mammals, critical for establishing and maintaining cell identity. ; Spanish Ministry of Economy co-funded by the EuropeanRegional Development Fund (ERDF) (SAF2013-48256-R), the EuropeanResearch Council (ERC-2014-AdG/669622), the Regional Government of Ma-drid co-funded by the European Social Fund (ReCaRe project), the EuropeanUnion (RISK-IR project), the Botin Foundation and Banco Santander(Santander Universities Global Division), the Ramon Areces Foundation, andthe AXA Foundation. S.R. was funded by a contract from the Ramon y CajalProgram(RYC-2011-09242) and by the Spanish Ministry of Economy co-funded by the ERDF (SAF2013-49147-P and SAF2016-80874-P). ; Peer reviewed

The RNA polymerase II-associated protein 1 (RPAP1) is conserved across metazoa and required for stem cell differentiation in plants; however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of cell identity genes and for cell viability. Depletion of RPAP1 triggers cell de-differentiation, facilitates reprogramming toward pluripotency, and impairs differentiation. Mechanistically, we show that RPAP1 is essential for the interaction between RNA polymerase II (RNA Pol II) and Mediator, as well as for the recruitment of important regulators, such as the Mediator-specific RNA Pol II factor Gdown1 and the C-terminal domain (CTD) phosphatase RPAP2. In agreement, depletion of RPAP1 diminishes the loading of total and Ser5-phosphorylated RNA Pol II on many genes, with super-enhancer-driven genes among the most significantly downregulated. We conclude that Mediator/RPAP1/RNA Pol II is an ancient module, conserved from plants to mammals, critical for establishing and maintaining cell identity. Lynch et al. report a regulator of RNA Pol II called RPAP1, displaying functional conservation from plants to mammals. RPAP1 is required to establish and maintain cell identity. Mechanistically, RPAP1 is critical for the Mediator-RNA Pol II interaction, thereby preserving normal transcription at enhancer-driven genes ; Work in the laboratory of M.S. is funded by the CNIO and the IRB and by grants from the Spanish Ministry of Economy co-funded by the European Regional Development Fund (ERDF) (SAF2013-48256-R), the European Research Council (ERC-2014-AdG/669622), the Regional Government of Madrid co-funded by the European Social Fund (ReCaRe project), the European Union (RISK-IR project), the Botin Foundation and Banco Santander (Santander Universities Global Division), the Ramon Areces Foundation, and the AXA Foundation. S.R. was funded by a contract from the Ramon y Cajal Program(RYC-2011-09242) and by the Spanish Ministry of Economy cofunded by the ERDF (SAF2013-49147-P and SAF2016-80874-P).

The RNA polymerase II-associated protein 1 (RPAP1) is conserved across metazoa and required for stem cell differentiation in plants; however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of cell identity genes and for cell viability. Depletion of RPAP1 triggers cell de-differentiation, facilitates reprogramming toward pluripotency, and impairs differentiation. Mechanistically, we show that RPAP1 is essential for the interaction between RNA polymerase II (RNA Pol II) and Mediator, as well as for the recruitment of important regulators, such as the Mediator-specific RNA Pol II factor Gdown1 and the C-terminal domain (CTD) phosphatase RPAP2. In agreement, depletion of RPAP1 diminishes the loading of total and Ser5-phosphorylated RNA Pol II on many genes, with super-enhancer-driven genes among the most significantly downregulated. We conclude that Mediator/RPAP1/RNA Pol II is an ancient module, conserved from plants to mammals, critical for establishing and maintaining cell identity. ; We are grateful to Elisa Varela for assistance with morula and blastocyst fixa- tion. Work in the laboratory of M.S. is funded by the CNIO and the IRB and by grants from the Spanish Ministry of Economy co-funded by the European Regional Development Fund (ERDF) (SAF2013-48256-R), the European Research Co uncil (ERC-2014-AdG/66 9622), the Region al Government of Ma- drid co-funded by the Euro pean Social Fund (ReCaRe project), the Euro pean Union (RISK-IR project), the Botin Foundation and Banco Santander (Santander Universities Glo bal Division), the Ramon Areces Found ation, and the AXA Foundation. S.R. was funded by a contract from the Ramon y Cajal Program(RYC-2011-09242) and by the Spanish Ministry of Economy co- funded by the ERDF (SAF2013-49147- P and SAF2016-80874-P ; Sí

Understanding the regulation of normal and malignant human hematopoiesis requires comprehensive cell atlas of the hematopoietic stem cell (HSC) regulatory microenvironment. Here, we develop a tailored bioinformatic pipeline to integrate public and proprietary single-cell RNA sequencing (scRNA-seq) datasets. As a result, we robustly identify for the first time 14 intermediate cell states and 11 stages of differentiation in the endothelial and mesenchymal BM compartments, respectively. Our data provide the most comprehensive description to date of the murine HSC-regulatory microenvironment and suggest a higher level of specialization of the cellular circuits than previously anticipated. Furthermore, this deep characterization allows inferring conserved features in human, suggesting that the layers of microenvironmental regulation of hematopoiesis may also be shared between species. Our resource and methodology is a stepping-stone toward a comprehensive cell atlas of the BM microenvironment. ; We would like to thank the staff of the flow cytometry core, the advances genomic lab, and the animal facility at CIMA Universidad de Navarra for their invaluable technical and intellectual assistance. We are particularly grateful to the healthy volunteers who donated bone marrow tissue for this study. We also acknowledge Ali O. Balubaid's help in writing. We would like to acknowledge Miguel Cocera-Fernandez's contribution to the Graphical Abstract. ; Funded by grants from The Spanish Government, through project PID2019-111192GA-I00 (MICINN) to DGC. Instituto de Salud Carlos III (ISCIII) and co-financed by FEDER: PI16/02024, PI17/00701 and PI19/01352, TRANSCAN EPICA AC16/00041, CIBERONC CB16/12/00489; Redes de Investigación Cooperativa (TERCEL RD16/0011/0005); Spanish Ministry of Economy, Industry and Competitivity (RTHALMY SAF2017-92632-EXP); Departamento de Salud, Gobierno de Navarra 40/2016 and Departamento de Desarrollo Económico y Empresarial (AGATA 0011-1411-2020-000010 and 0011-1411-2020-000013). The study was also ...

Cumulative toxicity from weekly paclitaxel (myalgia, peripheral neuropathy, fatigue) compromises long-term administration. Preclinical data suggest that the burden of critically short telomeres ( 21.9% CSTs) had 2-fold higher number of neuropathy (P = 0.04) or fatigue (P = 0.019) episodes and >3-fold higher number of myalgia episodes (P = 0.005). The average telomere length was unrelated to the incidence of side effects.The percentage of CSTs, but not the average telomere size, is associated with weekly paclitaxel-derived toxicity. ; This work was supported by the Fondo de Investigación Sanitaria [FIS PI10/00288 and FIS PI13/00430]; AECC Scientific Foundation [Beca de Retorno-2010, to MQF]; Spanish Ministry of Economy and Competitiveness Projects [SAF2013-45111-R]; Madrid Regional Government Projects [S2010/BMD- 2303]; AXA ...