In this study, the year-round protein, amino acid, fatty acid, pigments, mineral and vitamin content and profiles were considered to evaluate the nutritional value and harvest time of the Saccharina latissima biomass for optimized value and application. Sugarkelp was cultivated both in close proximity to a blue mussel and fish farm (IMTA) and in a reference site, both outside Horsens fjord in Denmark. Sugarkelp biomass was measured by harvesting sporophytes (deployed in February 2013) from 1m rope droppers (n=3) at 2 m depth in 2013-2014. Biomass was weighed, followed by freeze drying, homogenizing and chemical characterization by various methods for the specific analyses of biomass composition. Protein content varied throughout the experimental period with the highest values recorded in November (10.8%) and the lowest values recorded in May 2013 (1.3 %). The lipid concentration varied from 0.62-0.88% DW in July to 3.33-3.35% DW in November (P<0.05). Polyunsaturated fatty acids (PUFA's) made up more than half of the fatty acids with a maximum in July (52.3-54.0% FAME), including the most appreciated health beneficial EPA and DHA. Mineral content are discussed in relation to legislations, and a few trace metals such as Cd, Pb, Iodine may be problematic in some seasons. The pigment profile did not change during the year, however the concentration did, and with fucoxanthin as the most interesting. Generally the year-round variations were due season, and no difference between the two locations (reference and IMTA).
Seaweed represents an abundant, renewable, and fast-growing biomass resource for 3rd generation biofuel production. This study reports an efficient butanol fermentation process carried out by Clostridium beijerinckii DSM-6422 using enzymatic hydrolysate of the sugar-rich brown seaweed Laminaria digitata harvested from the coast of the Danish North Sea as substrate. The highest butanol yield (0.42 g/gconsumed-substrates) compared to literature was achieved, with a significantly higher butanol: acetone-butanol-ethanol (ABE) molar ratio (0.85) than typical (0.6). This demonstrates the possibility of using the seaweed L. digitata as a potential biomass for butanol production. For the first time, consumption of alginate components was observed by C. beijerinckii DSM-6422. The efficient utilization of sugars and lactic acid further highlighted the potential of using this strain for future development of large-scale cost-effective butanol production based on (ensiled) seaweed.