Suchergebnisse

2005 marks the tenth year since the ten countries of Southeast Asia declared the region a nuclear weapon-free zone. Under the new and fast evolving strategic environment, is the original purpose of establishing the zone still valid? This article will suggest that SEANWFZ remains strategically relevant because it conveys a message of peace, contributes to the global campaign against the proliferation of nuclear weapons, adds pressure to the Nuclear Weapon States to pursue nuclear disarmament and contributes to building the confidence of Southeast Asian countries in managing their common security and exercising a good measure of control over their destiny. It is also relevant to todays counter-terrorism efforts by ensuring that WMD materials, and technology from which nuclear weapons could be developed, do not fall into the hands of terrorists or be transmitted through the Zone. (Contemp Southeast Asia/DÜI)

According to the author, Southeast Asia today enjoys a state of relative stability and sustained economic growth. The ASEAN is at the forefront of these developments. Global and regional changes have served as imperatives for ASEAN to undertake a continuing review of its outlook and ways of realizing its outlook. Management experts refer to this process as "re-engineering". Borrowing this concept of change management, he explores how far ASEAN has re-engineered itself in terms of policy redirection and organizational change. (DÜI-Sen)

Recent studies have challenged the prevailing dogma that transcription is repressed during mitosis. Transcription was also proposed to sustain a robust spindle assembly checkpoint (SAC) response. Here, we used live-cell imaging of human cells, RNA-seq and qPCR to investigate the requirement for de novo transcription during mitosis. Under conditions of persistently unattached kinetochores, transcription inhibition with actinomycin D, or treatment with other DNA- intercalating drugs, delocalized the chromosomal passenger complex (CPC) protein Aurora B from centromeres, compromising SAC signaling and cell fate. However, we were unable to detect significant changes in mitotic transcript levels. Moreover, inhibition of transcription independently of DNA intercalation had no effect on Aurora B centromeric localization, SAC response, mitotic progression, exit or death. Mechanistically, we show that DNA intercalating agents reduce the interaction of the CPC with nucleosomes. Thus, mitotic progression, arrest, exit or death is determined by centromere structural integrity, rather than de novo transcription. ; We thank Jonathan Pines (ICR, London, UK), Ben Black and Michael Lampson (University of Pennsylvania, Pennsylvania, USA) for providing the cyclin B1-venus and LAP-Aurora B HeLa cells, respectively, Reto Gassmann (i3S, Porto, Portugal), Iain Cheeseman (MIT, Cambridge, USA), Andrea Musacchio (MIP, Dortmund, Germany), Jonathan Higgins (Institute for Cell and Molecular Biosciences, Newcastle, UK), Lars Jansen (IGC, Oeiras, Portugal) and Geert Kops (Hubrecht Institute, Utrecht, The Netherlands) for the generous gift of antibodies, Nathanael Gray (Harvard University, USA) for the gift of Mps1-IN-1, Paula Sampaio (i3S, Porto, Portugal) and André Maia (i3S, Porto, Portugal) for assistance with microscopy, Patricia Oliveira and Pedro Ferreira from Bioinf2Bio for bioinformatics and data analysis, and Bernard Orr for the critical reading of the manuscript and helpful suggestions. We also thank José Silva for inspiring discussions and CID Lab members for feedback during the course of this project. The authors declare no conflicting financial interests. MC holds a doctoral fellowship (SFRH/BD/117063/2016) and CF has an Investigator starting grant (IF/00765/2014) from Fun-dac¸ ão para a Ciência e a Tecnologia (FCT) of Portugal. This project has been funded by Norte-01– 0145-FEDER-000029 and Norte-07–0124-FEDER-000003 from Norte 2020 and EXPL/IF/00765/2014/ CP1241/CT0003 from FCT. Work in the laboratory of HM is funded by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No 681443) and FLAD Life Science 2020.

16‐17 July 2010, International AIDS Society's Workshop "Towards a Cure": HIV Reservoirs and Strategies to Control Them, Vienna, Austria