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SummaryGladiolus microcarpus G.J. Lewis is a striking, summer‐flowering cremnophyte, with a strongly weeping habit, endemic to the Drakensberg Mountain Centre in southern Africa. A description and details of its history, taxonomy, habitat and cultivation are given.

The growth and development of gladiolus, a cut flower widely used in decorations and commemorative dates, are influenced by the ambient temperature. Elevated temperatures shorten the growing cycle and accelerate flowering, while low temperatures delay development. Four gladiolus cultivars (White Goddess, Purple Flora, Green Star, Yester e Spic en Span) were evaluated for growth, development, and quality of floral stems, during autumn, in average temperatures of 24.31ºC. During the vegetative period, White Goddess presented the highest plant height. Entry into the reproductive stage R1 occurred between 56.81 and 65.41 days after planting and harvesting in R3 occurred between 63 and 77 days. Purple Flora stood out as the earliest cultivar and Spic en Span as the early. The gladiolus plants had a plant height greater than 90 cm and an inflorescence diameter greater than 5 mm at harvest, values within the required commercial standards. However, Green Star and Spic en Span had several rapiers below 8, an undesirable value. The cultivar White Goddess had the highest longevity, 6.75 days. The cultivation of gladiolus in regions with a Brazilian tropical climate allows a reduction in the cultivation cycle, and production of floral stems within commercialization standards.

PREMISE: Gladiolus palustris (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species. METHODS AND RESULTS: We utilized a 454 pyrosequencing approach to identify simple sequence repeat (SSR) regions in a microsatellite‐enriched library. Of all SSR regions, 46 were screened for specific PCR amplification, and 15 were found to be applicable in the target species. We found 1.62–3.08 alleles per population (effective alleles: 1.58–2.08) that indicated moderate to high genetic diversity values (0.28–0.44) in three pilot populations. Cross‐species amplification was less effective in G. imbricatus and G. tenuis. CONCLUSIONS: The primers reported here can be used for the population genetic characterization of G. palustris. They will help us to better understand the conservation genetics of this highly endangered species.

International audience ; PremiseGladiolus palustris (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species.Methods and ResultsWe utilized a 454 pyrosequencing approach to identify simple sequence repeat (SSR) regions in a microsatellite‐enriched library. Of all SSR regions, 46 were screened for specific PCR amplification, and 15 were found to be applicable in the target species. We found 1.62–3.08 alleles per population (effective alleles: 1.58–2.08) that indicated moderate to high genetic diversity values (0.28–0.44) in three pilot populations. Cross‐species amplification was less effective in G. imbricatus and G. tenuis.ConclusionsThe primers reported here can be used for the population genetic characterization of G. palustris. They will help us to better understand the conservation genetics of this highly endangered species.

International audience ; PremiseGladiolus palustris (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species.Methods and ResultsWe utilized a 454 pyrosequencing approach to identify simple sequence repeat (SSR) regions in a microsatellite‐enriched library. Of all SSR regions, 46 were screened for specific PCR amplification, and 15 were found to be applicable in the target species. We found 1.62–3.08 alleles per population (effective alleles: 1.58–2.08) that indicated moderate to high genetic diversity values (0.28–0.44) in three pilot populations. Cross‐species amplification was less effective in G. imbricatus and G. tenuis.ConclusionsThe primers reported here can be used for the population genetic characterization of G. palustris. They will help us to better understand the conservation genetics of this highly endangered species.

International audience ; PremiseGladiolus palustris (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species.Methods and ResultsWe utilized a 454 pyrosequencing approach to identify simple sequence repeat (SSR) regions in a microsatellite‐enriched library. Of all SSR regions, 46 were screened for specific PCR amplification, and 15 were found to be applicable in the target species. We found 1.62–3.08 alleles per population (effective alleles: 1.58–2.08) that indicated moderate to high genetic diversity values (0.28–0.44) in three pilot populations. Cross‐species amplification was less effective in G. imbricatus and G. tenuis.ConclusionsThe primers reported here can be used for the population genetic characterization of G. palustris. They will help us to better understand the conservation genetics of this highly endangered species.