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The transcription factor p63 is a master regulator of ectoderm development. Although previous studies show that p63 triggers epidermal differentiation in vitro, the roles of p63 in developing embryos remain poorly understood. Here, we use zebrafish embryos to analyze in vivo how p63 regulates gene expression during development. We generate tp63-knock-out mutants that recapitulate human phenotypes and show down-regulated epidermal gene expression. Following p63-binding dynamics, we find two distinct functions clearly separated in space and time. During early development, p63 binds enhancers associated to neural genes, limiting Sox3 binding and reducing neural gene expression. Indeed, we show that p63 and Sox3 are co-expressed in the neural plate border. On the other hand, p63 acts as a pioneer factor by binding non-accessible chromatin at epidermal enhancers, promoting their opening and epidermal gene expression in later developmental stages. Therefore, our results suggest that p63 regulates cell fate decisions during vertebrate ectoderm specification. ; This work was supported by a grant from the Spanish Ministry of Economy and Competitiveness to J.J.T. (BFU2014-58449-JIN) and an ERC-Advanced grant from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 740041). J.M.S.-P. was funded by a postdoctoral contract associated to an Excellence Project from Junta de Andalucía (P12-BIO-396) and by a Juan de la Cierva-Incorporación fellow from the Spanish Ministry of Economy and Competitiveness (IJCI-2016-29884).

This paper discusses evidence from field studies undertaken to investigate the responsiveness of the order fulfilment process in a number of companies. The evidence is analysed in the context of the literature on responsiveness and related areas such as time‐based competition. Similarities and differences are analysed across a number of industrial sectors with respect to order fulfilment processes and the interpretation and significance of responsiveness. Generic factors that influence different types of companies are identified. Four components of responsiveness – stimuli, awareness, capabilities and goals – emerge from an analysis of the literature. The field and case study evidence allows the development of more precise definitions and descriptions of each of these components. The study also allows a generic responsiveness framework to be developed that incorporates both strategic and operational viewpoints. The need for more field studies on responsiveness is noted. More work is advocated on the assessment and measurement of responsiveness and on developing appropriate responsiveness interventions, particularly with respect to the order fulfilment process.

The main question for consideration in this paper relates to the functional prerequisites for the construction of the so-called 'digital family' in the Spanish context. Taking inspiration from Burman's (1994; 2008) work we start from two assumptions. Firstly, we assume that representations of children and families are important in terms of power and gender. This leads to our second assumption, that when considering representations of the family we also, necessarily, need to address gender regulatory functions, as much as economic and political trends. We argue that since the middle of the last decade reports there has been an intensification of interest in children and their relations with Information and Communications Technology. We identify how a convergence of discourses around family and gender, enshrined in notions of the 'digital family' functions to refold the family structure back in on itself, advocating a return to traditional values in much the same way as in previous eras.

PurposeThe purpose of this paper is to present an empirical study of the link between psychological contract fulfilment and affective states at work. The paper argues that perceived organizational support is the key attitudinal intervening variable that arises from the cognitive assessment of the exchange relationship between employer and employee and is in turn related to the generation of affective states at work.Design/methodology/approachThe paper tests this assumption using a manager sample of 249 participants and a longitudinal design.FindingsPerceived organizational support mediates the relationship between psychological contract fulfilment and workplace affect.Research limitations/implicationsAffect was not measured in real time, but through self‐reports. Future research could study how and under what conditions psychological contract fulfilment generates perceived organizational support.Originality/valueOne of the few studies that have sought to research the affective dimension of the psychological contract.