Molecular insights on potato yellow vein crinivirus infections in the highlands of Colombia
12 p.-6 fig.-2 tab. ; Potato yellow vein virus (PYVV) was detected in potatoes grown in the Central highlands, north of Bogotá (~3000 m altitude),Colombia. At this altitude viral whitefly vectors are largely absent, but infection persists because of the use of uncertified tubers.Plants with typical PYVV-induced yellowing symptoms, as well as with atypical yellowing or non-symptomatic symptoms were sampled at three separate geographical locations. PYVV presence was assessed by RT-PCR,and several plants were subjected to high-throughput sequencing (HTS) of their small RNA (sRNA) populations. Complete or almost complete sequences of four PYVV isolates were thus reconstructed, all from symptomatic plants. Three viral isolates infected plants singly, while the fourth co-infected the plant together with a potyvirus. Relative proportions of sRNAs to each of the three crinivirus genomic RNAs were found to remain comparable among the four infections. Genomic regions were identified as hotspots of sRNA formation,or as regions that poorly induced sRNAs. Furthermore, PYVV titres in the mixed versus single infections remained comparable,indicating an absence of synergistic/antagonistic effects of the potyvirus on the accumulation of PYVV. Daughter plants raised in the greenhouse from tubers of the infected, field-sampled plants displayed mild PYVV infection symptoms that disappeared with time, demonstrating the occurrence of recovery and asymptomatic infection phenotypes in this pathosystem. ; This work was funded by Grants PID2019-109304RB-I00 and COOPB20310 from the 2019 and 2017 calls by the Ministry of Science and Innovation of the Government of Spain, and the Spanish Council for Scientific Research; and by Grant CIAS 2742 from the Universidad Militar Nueva Granada, Colombia. ; Peer reviewed