Suchergebnisse

Muhammad Hanif,1 Shahid Shah,2 Akhtar Rasul,3 Ghulam Abbas,3 Muhammad Zaman,4 Muhammad Wahab Amjad,5 Maria Abdul Ghafoor Raja,5 Hafeez Ullah Khan,6 Mehran Ashfaq,3 Omeira Iqbal3 1Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan; 2Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Pakistan; 3Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Pakistan; 4Faculty of Pharmacy, University of Central Punjab, Lahore, Pakistan; 5Department of Pharmaceutics, Faculty of Pharmacy, Northern Border University, Rafha, Saudi Arabia; 6College of Pharmacy, University of Sargodha, Sargodha, PakistanCorrespondence: Ghulam AbbasDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, PakistanEmail ghulamabbas@gcuf.edu.pkBackground: Bisphosphonates have very low bioavailability and cause irritation of the esophagus and stomach. This study was planned to improve the oral bioavailability of ibandronate through the formation of a raft in the stomach. Bisphosphonate-induced irritation of the esophagus and stomach is prevented by the formation of a raft.Materials and Methods: The nanostructured raft was developed through the use of nanosized citrus pectin (NCP). The particle size of NCP was measured by zeta sizer and SEM. The percentage of NCP and the neutralization profile of raft was studied. The ibandronate, polymers, and the developed formulation were characterized by FTIR, XRD, TGA, and DSC. The release of ibandronate was studied in 0.1 N HCl, 0.5 N HCl, 1 N HCl, and simulated gastric fluid (SGF) and a cell viability study was performed using Caco-2 cells. The PPR5 formulation and Bonish 150 mg tablets were selected as test and reference formulations, respectively, for pharmacokinetic study. Twelve healthy albino rats were taken and divided into two groups using a Latin square crossover design, and the blood samples were collected for 24 hours.Results: The SEM image showed that the particle size of NCP was 159 nm. The raft of PPR5 showed 94% NCP and 45 minutes duration of neutralization. The FTIR and XRD showed chemical stability and a uniform distribution of ibandronate in the raft. The TGA and DSC indicated the thermal stability of formulation. The release of 99.87% ibandronate at 20 minutes was observed in the SGF. The values of Cmax for the reference and test formulations were 493± 0.237 ng/mL and 653± 0.097 ng/mL, respectively. The AUC(0-t) of the reference and test formulations was 3708.25± 3.418 ng/mL.h and 6899.25± 3.467 ng/mL.h, respectively.Conclusion: The NCP has been successfully prepared from citrus pectin and has shown effective porous raft formation. The bioavailability of the ibandronate from newly developed PPR5 was higher than the already marketed formulation.Keywords: nanosized citrus pectin, raft, in vitro release, cell viability, pharmacokinetics

Shahid Shah,1 Muhammad Hanif,2 Hafeez Ullah Khan,3 Faiz Ullah Khan,4 Ghulam Abbas,5 Haris Khurram,6 Ahmed Khames,7 Mohamed A Abdelgawad,8 Amira SA Said,9,10 Mohammed AS Abourehab,11 Safirah Maheen,3 Usman Rashid Chand,1 Muhammad Haris1 1Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, Pakistan; 2Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University Multan, Multan, Pakistan; 3College of Pharmacy, Department of Pharmaceutics, University of Sargodha, Sargodha, Pakistan; 4Department of Pharmacy Administration and Clinical Pharmacy, School of Pharmacy, Xi'an Jiaotong University, Xi'an, People's Republic of China; 5Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, Pakistan; 6National University of Computer and Emerging Sciences, Chiniot-Faisalabad Campus, Chiniot, Pakistan; 7Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University, Taif, 21944, Saudi Arabia; 8Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka, Al Jouf, 72341, Saudi Arabia; 9Clinical Pharmacy Department, Faculty of Pharmacy, Beni-Suef, Egypt; 10Clinical Pharmacy Department, College of Pharmacy, Al Ain University, Al Ain, Abu Dhabi, United Arab Emirates; 11Department of Pharmaceutics, Faculty of Pharmacy, Umm Al-Qura University, Makkah, 21955, Saudi ArabiaCorrespondence: Shahid Shah; Ghulam Abbas, Email Shahid.waris555@gmail.com; ghulamabbas@gcuf.edu.pkBackground: Pharmacogenomics (PGx) is essential for optimizing drug therapy and reducing unwanted drug side effects. Our aim was to determine the knowledge, attitude and practice of senior pharmacy students in Punjab, Pakistan towards PGx.Methods: A cross-sectional study was conducted among 511 undergraduate pharmacy students from different universities in Punjab, Pakistan. A validated and pilot-tested structured questionnaire was administered to respondents to assess their knowledge, attitude, and practice regarding PGx. Descriptive statistics and multivariate logistic regression model were used to describe the results of the study. A p-value of less than 0.05 was considered statistically significant.Results: A total of 511 students (58.9% females and 41.1% males) responded to the survey. Most (87.7%) of the students knew that the drug response could be affected by genetic variations. Mean knowledge score of the students was 12.6. Good basic knowledge positively and significantly (p=0.01) affected students' PGx test knowledge and attitudes. Most (> 70%) of students believed that human genetics affected the drug response due to interindividual variation and ethnic variation. Mean attitude score of the students was 5.23. The majority of students (> 92%) wanted to learn more about PGx and thought it could help them choose the right drug. Students' knowledge was positively and significantly (p=0.01) affected by a good attitude. Mean practice score of the students was 11.95. When trying to solve a drug-related case study question, about 31.5% of students implemented the idea of human genetic variation. Only 28.8% of students attended a lecture related to the effects of genetic variations on drug therapy. Good practice positively and significantly (p=0.01) affected students' knowledge of PGx tests.Conclusion: The senior pharmacy students had good knowledge and attitudes towards PGx. However, the practice of PGx needs to be improved to facilitate the application of PGx in clinical practice in Pakistan.Keywords: pharmacogenomics, knowledge, attitude, practice