AbstractThis study examines how patients use narratives to evaluate their experiences of healthcare services online. The analysis draws on corpus linguistic techniques, specifically annotation, applyingLabov and Waletzky's (1967)framework to a sample of online comments about the NHS in England. Narratives are pervasive in this context, being present more than absent in the patients' comments, but are particularly prominent in comments which evaluate care negatively. Evaluations can be accomplished through all the structural elements of the narrative, including in combination with one another. However, the presence and ordering of these elements does not seem to be influenced by the type of evaluation given (i.e. positive, negative or more neutral). As mediated social practice, the narratives are shaped by the technological affordances and social dynamics of this context, for instance in the placement of particular structural elements and the design of narratives for particular "imagined" audiences.
We thank the Macaulay Development Trust and School of Geosciences, University of Aberdeen for KDP's scholarship. JG would like to acknowledge funding from the Royal Society (RG140402), the Carnegie Trust for the Universities of Scotland (project 70112) and the UK Natural Environment Research Council (project NE/N007611/1 and CC13_080). RR received funding from the Natural Environment Research Council (projects NE/M003086/1, NE/ R004897/1 and NE/T005645/1) and from the International Atomic Energy Agency of the United Nations (IAEA/UN) (project CRP D12014). MW and AL were funded by the Rural & Environment Science & Analytical Services Division of the Scottish Government. We thank Lucile Verrot for helping with the depth-distance weighting of soil moisture. We thank to Hydroinnova for making available the solar intensity data from Jungfraujoch to us. Special thanks to Jessica Fennell, Lucile Verrot and Mark Grundy for assistance with fieldwork as well as to David Finlay and his team for enabling land access in Elsick. ; Peer reviewed ; Postprint
Acknowledgements We thank the Macaulay Development Trust and School of Geosciences, University of Aberdeen for KDPs scholarship. JG would like to acknowledge funding from the Royal Society and the Carnegie Trust for the Universities of Scotland (project 70112). JG and LV acknowledge funding from the UK Natural Environment Research Council (project NE/N007611/1 and CC13_080). MW was supported by the Rural & Environment Science & Analytical Services Division of the Scottish Government. RR received funding from the Natural Environment Research Council (projects NE/M003086/1, NE/R004897/1 and NE/T005645/1) and from the International Atomic Energy Agency of the United Nations (IAEA/UN) (project CRP D12014). Special thanks to Carol Taylor, Jessica Fennell, Alice Poli and many more for assistance with fieldwork. Finally, we would like to acknowledge Kenneth Loades for providing us with essential equipment for soil sampling and thank David Finlay and his team for enabling land access in Elsick. ; Peer reviewed ; Postprint
Transition type dwarf galaxies are thought to be systems undergoing the process of transformation from a star-forming into a passively evolving dwarf, which makes them particularly suitable to study evolutionary processes driving the existence of different dwarf morphological types. Here we present results from a spectroscopic survey of ∼200 individual red giant branch stars in the Phoenix dwarf, the closest transition type with a comparable luminosity to "classical" dwarf galaxies. We measure a systemic heliocentric velocity Vhelio = −21.2 ± 1.0 km s−1. Our survey reveals the clear presence of prolate rotation, which is aligned with the peculiar spatial distribution of the youngest stars in Phoenix. We speculate that both features might have arisen from the same event, possibly an accretion of a smaller system. The evolved stellar population of Phoenix is relatively metal-poor ( = − 1.49 ± 0.04 dex) and shows a large metallicity spread (σ[Fe/H] = 0.51 ± 0.04 dex), with a pronounced metallicity gradient of −0.13 ± 0.01 dex per arcmin similar to luminous, passive dwarf galaxies. We also report a discovery of an extremely metal-poor star candidate in Phoenix and discuss the importance of correcting for spatial sampling when interpreting the chemical properties of galaxies with metallicity gradients. This study presents a major leap forward in our knowledge of the internal kinematics of the Phoenix transition type dwarf galaxy, and the first wide area spectroscopic survey of its metallicity properties. ; This study was partially financially supported by a 2015 ESO DGDF grant. NK acknowledges financial support from IAC for a three-weeks visit to the institute. GB gratefully acknowledges support through a Marie- Curie action Intra European Fellowship, funded by the European Union Seventh Framework Program (FP7/2007-2013) under Grant agreement number PIEF-GA-2010-274151, as well as the financial support by the Spanish Ministry of Economy and Competitiveness (MINECO) under the Ramon y Cajal Programme (RYC-2012-11537). AC was supported by a fellowship from the Netherlands Research School for Astronomy (NOVA). This work made extensive use of the NASA Astrophysics Data System bibliographic services. This research used the facilities of the Canadian Astronomy Data Centre operated by the National Research Council of Canada with the support of the Canadian Space Agency. ; Peer-reviewed ; Post-print
BACKGROUND: Skin diseases constitute up to 40% of all notified occupational diseases in most European countries, predominantly comprising contact dermatitis, contact urticaria, and skin cancer. While insufficient prevention of work-related skin diseases (WRSD) is a top-priority problem in Europe, common standards for prevention of these conditions are lacking. OBJECTIVE: To develop common European standards on prevention and management of WRSD and occupational skin diseases (OSD). METHOD: Consensus amongst experts within occupational dermatology was achieved with regard to the definition of minimum evidence-based standards on prevention and management of WRSD/OSD. RESULTS: By definition, WRSDs/OSDs are (partially or fully) caused by occupational exposure. The definition of OSD sensu stricto additionally includes diverging national legal requirements, with an impact on registration, prevention, management, and compensation. With the implementation of the classification of WRSD/OSD in the International Classification of Diseases (ICD) 11th Revision in future, a valid surveillance and comparability across countries will be possible. Currently, WRDS and OSD are still under-reported. Depending on legislation and regulations, huge differences exist in notification procedures in Europe, although notification is crucial to prevent chronic and relapsing disease. Facilities for early diagnosis, essential for individual patient management, should be based on existing guidelines and include a multidisciplinary approach. Patch testing is essential if contact dermatitis persists or relapses. Workplace exposure assessment of WRSD/OSD requires full labelling of product ingredients on material safety data sheets helping to identify allergens, irritants and skin carcinogens. Comparable standards in primary, secondary and tertiary prevention must be established in Europe to reduce the burden of WRSD/OSD in Europe. CONCLUSION: The adoption of common European standards on prevention of WRSD/OSD will contribute to reduce the incidence of OSD and their socio-economic burden.
High-quality and complete reference genome assemblies are fundamental for the application of genomics to biology, disease, and biodiversity conservation. However, such assemblies are available for only a few non-microbial species1,2,3,4. To address this issue, the international Genome 10K (G10K) consortium5,6 has worked over a five-year period to evaluate and develop cost-effective methods for assembling highly accurate and nearly complete reference genomes. Here we present lessons learned from generating assemblies for 16 species that represent six major vertebrate lineages. We confirm that long-read sequencing technologies are essential for maximizing genome quality, and that unresolved complex repeats and haplotype heterozygosity are major sources of assembly error when not handled correctly. Our assemblies correct substantial errors, add missing sequence in some of the best historical reference genomes, and reveal biological discoveries. These include the identification of many false gene duplications, increases in gene sizes, chromosome rearrangements that are specific to lineages, a repeated independent chromosome breakpoint in bat genomes, and a canonical GC-rich pattern in protein-coding genes and their regulatory regions. Adopting these lessons, we have embarked on the Vertebrate Genomes Project (VGP), an international effort to generate high-quality, complete reference genomes for all of the roughly 70,000 extant vertebrate species and to help to enable a new era of discovery across the life sciences. ; We thank them for their permission to publish. A.R., S.K., B.P.W. and A.M.P. were supported by the Intramural Research Program of the NHGRI, NIH (1ZIAHG200398). A.R. was also supported by the Korea Health Technology R&D Project through KHIDI, funded by the Ministry of Health & Welfare, Republic of Korea (HI17C2098). S.A.M., I.B. and R.D. were supported by Wellcome Trust grant WT207492; W.C., M. Smith, Z.N., Y.S., J.C., S. Pelan, J.T., A.T., J.W. and Kerstin Howe by WT206194; L.H., F.M., Kevin Howe and P. Flicek by WT108749/Z/15/Z, WT218328/B/19/Z and the European Molecular Biology Laboratory. O.F. and E.D.J. were supported by Howard Hughes Medical Institute and Rockefeller University start-up funds for this project. J.D. and H.A.L. were supported by the Robert and Rosabel Osborne Endowment. M.U.-S. received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement (750747). F.T.-N., J. Hoffman, P. Masterson and K.C. were supported by the Intramural Research Program of the NLM, NIH. C.L., B.J.K., J. Kim and H.K. were supported by the Marine Biotechnology Program of KIMST, funded by the Ministry of Ocean and Fisheries, Republic of Korea (20180430). M.C. was supported by Sloan Research Fellowship (FG-2020-12932). S.C.V. was funded by a Max Planck Research Group award from the Max Planck Society, and a Human Frontiers Science Program (HFSP) Research grant (RGP0058/2016). T.M.L., W.E.J. and the Canada lynx genome were funded by the Maine Department of Inland Fisheries & Wildlife (F11AF01099), including when W.E.J. held a National Research Council Research Associateship Award at the Walter Reed Army Institute of Research (WRAIR). C.B. was supported by the NSF (1457541 and 1456612). D.B. was funded by The University of Queensland (HFSP - RGP0030/2015). D.I. was supported by Science Exchange Inc. (Palo Alto, CA). H.W.D. was supported by NSF grants (OPP-0132032 ICEFISH 2004 Cruise, PLR-1444167 and OPP-1955368) and the Marine Science Center at Northeastern University (416). G.J.P.N. and the thorny skate genome were funded by Lenfest Ocean Program (30884). M.P. was funded by the German Federal Ministry of Education and Research (01IS18026C). M. Malinsky was supported by an EMBO fellowship (ALTF 456-2016). The following authors' contributions were supported by the NIH: S. Selvaraj (R44HG008118); C.V.M., S.R.F., P.V.L. (R21 DC014432/DC/NIDCD); K.D.M. (R01GM130691); H.C. (5U41HG002371-19); M.D. (U41HG007234); and B.P. (R01HG010485). D.G. was supported by the National Key Research and Development Program of China (2017YFC1201201, 2018YFC0910504 and 2017YFC0907503). F.O.A. was supported by Al-Gannas Qatari Society and The Cultural Village Foundation-Katara, Doha, State of Qatar and Monash University Malaysia. C.T. was supported by The Rockefeller University. M. Hiller was supported by the LOEWE-Centre for Translational Biodiversity Genomics (TBG) funded by the Hessen State Ministry of Higher Education, Research and the Arts (HMWK). H.C. was supported by the NHGRI (5U41HG002371-19). R.H.S.K. was funded by the Max Planck Society with computational resources at the bwUniCluster and BinAC funded by the Ministry of Science, Research and the Arts Baden-Württemberg and the Universities of the State of Baden-Württemberg, Germany (bwHPC-C5). B.V. was supported by the Biomedical Research Council of A*STAR, Singapore. T.M.-B. was funded by the European Research Council under the European Union's Horizon 2020 research and innovation programme (864203), MINECO/FEDER, UE (BFU2017-86471-P), Unidad de Excelencia María de Maeztu, AEI (CEX2018-000792-M), a Howard Hughes International Early Career award, Obra Social "La Caixa" and Secretaria d'Universitats i Recerca and CERCA Programme del Departament d'Economia i Coneixement de la Generalitat de Catalunya (GRC 2017 SGR 880). E.C.T. was supported by the European Research Council (ERC-2012-StG311000) and an Irish Research Council Laureate Award. M.T.P.G. was supported by an ERC Consolidator Award 681396-Extinction Genomics, and a Danish National Research Foundation Center Grant (DNRF143). T.W. was supported by the NSF (1458652). J. M. Graves was supported by the Australian Research Council (CEO561477). E.W.M. was partially supported by the German Federal Ministry of Education and Research (01IS18026C). Complementary sequencing support for the Anna's hummingbird and several genomes was provided by Pacific Biosciences, Bionano Genomics, Dovetail Genomics, Arima Genomics, Phase Genomics, 10X Genomics, NRGene, Oxford Nanopore Technologies, Illumina, and DNAnexus. All other sequencing and assembly were conducted at the Rockefeller University, Sanger Institute, and Max Planck Institute Dresden genome labs. Part of this work used the computational resources of the NIH HPC Biowulf cluster (https://hpc.nih.gov). We acknowledge funding from the Wellcome Trust (108749/Z/15/Z) and the European Molecular Biology Laboratory. ; With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2018-000792-M). ; Peer reviewed
