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This exploratory study examines identity exploration and commitment in different domains and how family structure (original/alternative), gender, and age affect these processes in a convenience sample of 388 college students. Results reveal that participants from alternative family structures explore more in the political and gender role domains than do participants from original families. Furthermore, women explore more in the occupational and friendship domains and are more committed in the values and family domains. Younger participants explore less in the gender role domain compared to older ones. Significant interactions reveal that (a) younger women explored the least, and (b) men from alternative families are the least committed in the values domain, compared to all other groups. Findings support the value of exploring the relative impact of family-focused experience on the processes of identity formation and, for the current convenience sample, suggest that growing up in an alternative family structure does not necessarily have a negative impact on identity.

15 páginas, 6 figuras, material suplementario ; Developmentally Regulated GTP-binding (DRG) proteins are highly conserved GTPases that associate with DRG Family Regulatory Proteins (DFRP). The resulting complexes have recently been shown to participate in eukaryotic translation. The structure of the Rbg1 GTPase, a yeast DRG protein, in complex with the C-terminal region of its DFRP partner, Tma46, was solved by X-ray diffraction. These data reveal that DRG proteins are multimodular factors with three additional domains, helix-turn-helix (HTH), S5D2L and TGS, packing against the GTPase platform. Surprisingly, the S5D2L domain is inserted in the middle of the GTPase sequence. In contrast, the region of Tma46 interacting with Rbg1 adopts an extended conformation typical of intrinsically unstructured proteins and contacts the GTPase and TGS domains. Functional analyses demonstrate that the various domains of Rbg1, as well as Tma46, modulate the GTPase activity of Rbg1 and contribute to the function of these proteins in vivo. Dissecting the role of the different domains revealed that the Rbg1 TGS domain is essential for the recruitment of this factor in polysomes, supporting further the implication of these conserved factors in translation ; Postdoctoral fellowship from the Ministerio de Ciencia e Innovación and Conselleria d'Educació, Generalitat Valenciana (to M.E.G.); Ministerio de Ciencia e Innovación [SAF2008-04048-E, SAF2009-10667 to J.B.]; Conselleria de Sanitat, Generalitat Valenciana [AP-001/10 and ACOMP/2012/039 to J.B.]; CSIC [200820I020 to J.B.]; European Union 6th Framework programs 3D Repertoire [contract LSHG-CT-2005-512028 to J.B. and B.S.]; CERBM-IGBMC (to B.S.); CNRS (to B.S.); Ligue Contre le Cancer [Equipe Labellisée 2011 to B.S.]. Funding for open access charge: CERBM GIE ; Peer reviewed

Cover -- Half Title -- Title Page -- Copyright Page -- Original Title Page -- Original Copyright Page -- Table of Contents -- List of Tables and Figures -- Acknowledgements -- Foreword -- 1 Theorising International Labour -- Introduction -- The Industrial Relations Tradition -- The Orthodox Left Tradition -- The New International Labour Studies -- The Themes of this Book -- Conclusion -- 2 Workers in Developing Societies -- Definitions -- Misconceptions -- Rural Workers -- Urban Workers -- Politics -- 3 Work, Culture and the Dialectics of Proletarian Habituation -- Introduction -- Exterior Conditioning -- Interior Determination: Early Phase -- Interior Determination: Later Phases -- Family and Community -- Habituation: A Continuing Issue -- Conclusion -- 4 The Control and Habituation of Agricultural Workers in the US -- Introduction -- The Role of the Government -- The Role of the Growers -- Interior Determination -- Habituation and Resistance -- 5 Peasants to Workers and Peasant-Workers in Africa -- Introduction -- The Emergence of Wage Labour -- Migrant Labour -- The Rural Proletariat -- Peasants as Proletarians? -- The Unskilled Urban Worker -- Managers, Professionals and Bureaucrats -- Other Urban Migrants -- Conclusion -- 6 Resistance and Hidden Forms of Consciousness Amongst African Workers -- Introduction -- The Labour Process and Worker Responses -- Worker Responses in Africa -- Hidden and Overt Forms -- 7 The Revolutionary Potential of the 'Lumpenproletariat': A Sceptical View from Africa -- Marginality? -- Romanticisation -- Coherence -- Identification of Class Enemy -- 'False Consciousness' -- The Political Dimension -- Conclusion -- 8 The 'New' International Division of Labour: A Conceptual, Historical and Empirical Critique -- Conceptual Problems -- Historical Gaps -- Empirical Omissions -- Conclusion.

This article examines changes in the degree of personal agency in young women, with post–compulsory education, in the last 50 years in a Southern European reality. It explores strategies of negotiation and resistance to social and cultural conditions, such as restrictive legislation, in a relatively traditional context. The arguments brought forward are positioned broadly within a discourse of individualization—on how women are more than ever devising their lives on their own free-will. There are various structural and cultural changes that had direct impact on the changes in females' personal agency. This article focuses on three of them—the influence of the church, restrictive legislation, and the expansion and extension of the educational system. The data drawn on for this article are taken from interviews conducted in Malta, with two generations of women who were in post–compulsory education in their youth, yet experienced their youth 50 years apart. The implications brought forward include a need for assessing how female agency operates in a relatively more traditional setting in a paradoxical manner. Their degree of female agency demonstrates complex strategies of accommodation and negotiation in line with socioeconomic and cultural conditions.

This article examines changes in the degree of personal agency in young women, with post–compulsory education, in the last 50 years in a Southern European reality. It explores strategies of negotiation and resistance to social and cultural conditions, such as restrictive legislation, in a relatively traditional context. The arguments brought forward are positioned broadly within a discourse of individualization—on how women are more than ever devising their lives on their own free-will. There are various structural and cultural changes that had direct impact on the changes in females' personal agency. This article focuses on three of them—the influence of the church, restrictive legislation, and the expansion and extension of the educational system. The data drawn on for this article are taken from interviews conducted in Malta, with two generations of women who were in post–compulsory education in their youth, yet experienced their youth 50 years apart. The implications brought forward include a need for assessing how female agency operates in a relatively more traditional setting in a paradoxical manner. Their degree of female agency demonstrates complex strategies of accommodation and negotiation in line with socioeconomic and cultural conditions. ; peer-reviewed

Abstract. This paper1 compares the structure of policy networks in two different policy domains. Policy networks are seen as clusters of relatively autonomous but interdependent actors that are incorporated into the process of public policy making. Policy networks have to be seen as specific actor configurations beyond 'policy markets'and 'policy hierarchies'.2 Their emergence is seen as a response to an increasing societal dispersion of resources, policy growth and governmental overload. Growing governmental activities in a context of more complex policy problems and a greater dispersion of policy resources within society makes governments increasingly dependent upon the horizontal cooperation of private actors in policy formulation and implementation. Institutional devices facilitating this mode of political resource mobilization range from formal advisory bodies, semi‐institutionalized working groups to highly informal and even 'secret'forms of cooptation of private actors (organizations and individuals) in the 'production process'of a policy.

Identifying conditions under which parents thrive is a key concern of family research. Prior research often focused on mothers' well-being in single life domains, yet it is more likely to be shaped by stressors that stem directly from the parenting role and related stressors emerging from spillover processes into other domains. We therefore examine how stressors concerning mothers' subjective, relational, and financial well-being accumulate and combine within subgroups of mothers and whether the likelihood to belong to these multidimensional subgroups varies by family structure. Using representative German data ( N = 11,242), latent class analysis revealed four distinct subgroups of maternal well-being with varying exposure to financial, psychological, and relational stressors. Regression models showed that particularly single mothers were at risk to belong to the most vulnerable group with exposure to multiple stressors. Findings are discussed in light of persisting disparities among post-separation families despite demographic trends toward growing family diversity.

The SH3 domain of the c-Src tyrosine kinase (c-Src-SH3) aggregates to form intertwined dimers and amyloid fibrils at mild acid pHs. In this work, we show that a single mutation of residue Gln128 of this SH3 domain has a significant effect on: (i) its thermal stability; and (ii) its propensity to form amyloid fibrils. The Gln128Glu mutant forms amyloid fibrils at neutral pH but not at mild acid pH, while Gln128Lys and Gln128Arg mutants do not form these aggregates under any of the conditions assayed. We have also solved the crystallographic structures of the wild-type (WT) and Gln128Glu, Gln128Lys and Gln128Arg mutants from crystals obtained at different pHs. At pH 5.0, crystals belong to the hexagonal space group P6522 and the asymmetric unit is formed by one chain of the protomer of the c-Src-SH3 domain in an open conformation. At pH 7.0, crystals belong to the orthorhombic space group P212121, with two molecules at the asymmetric unit showing the characteristic fold of the SH3 domain. Analysis of these crystallographic structures shows that the residue at position 128 is connected to Glu106 at the diverging β-turn through a cluster of water molecules. Changes in this hydrogen-bond network lead to the displacement of the c-Src-SH3 distal loop, resulting also in conformational changes of Leu100 that might be related to the binding of proline rich motifs. Our findings show that electrostatic interactions and solvation of residues close to the folding nucleation site of the c-Src-SH3 domain might play an important role during the folding reaction and the amyloid fibril formation. ; This research was funded by the Spanish Ministry of Science and Innovation and Ministry of Economy and Competitiveness and FEDER (EU): BIO2009-13261-C02-01/02 (ACA); BIO2012-39922-C02-01/02 (ACA); CTQ2013-4493 (JLN) and CSD2008-00005 (JLN); Andalusian Regional Government (Spain) and FEDER (EU): P09-CVI-5063 (ACA); and Valentian Regional Government (Spain) and FEDER (EU): Prometeo 2013/018 (JLN). Data collection was supported by European Synchrotron Radiation Facility (ESRF), Grenoble, France: BAG proposals MX-1406 (ACA) and MX-1541 (ACA); and ALBA (Barcelona, Spain) proposals 2012010072 (ACA) and 2012100378 (ACA).

15 pags, 8 figs ; Murine adenovirus 2 (MAdV-2) infects cells of the mouse gastrointestinal tract. Like human adenoviruses, it is a member of the genus Mastadenovirus, family Adenoviridae. The MAdV-2 genome has a single fibre gene that expresses a 787 residue-long protein. Through analogy to other adenovirus fibre proteins, it is expected that the carboxy-terminal virus-distal head domain of the fibre is responsible for binding to the host cell, although the natural receptor is unknown. The putative head domain has little sequence identity to adenovirus fibres of known structure. In this report, we present high-resolution crystal structures of the carboxy-terminal part of the MAdV-2 fibre. The structures reveal a domain with the typical adenovirus fibre head topology and a domain containing two triple ß-spiral repeats of the shaft domain. Through glycan microarray profiling, saturation transfer difference nuclear magnetic resonance spectroscopy, isothermal titration calorimetry and site-directed mutagenesis, we show that the fibre specifically binds to the monosaccharide N-acetylglucosamine (GlcNAc). The crystal structure of the complex reveals that GlcNAc binds between the AB and CD loops at the top of each of the three monomers of the MAdV-2 fibre head. However, infection competition assays show that soluble GlcNAc monosaccharide and natural GlcNAc-containing polymers do not inhibit infection by MAdV-2. Furthermore, site-directed mutation of the GlcNAc-binding residues does not prevent the inhibition of infection by soluble fibre protein. On the other hand, we show that the MAdV-2 fibre protein binds GlcNAc-containing mucin glycans, which suggests that the MAdV-2 fibre protein may play a role in viral mucin penetration in the mouse gut. ; This research was sponsored by grant BFU2014-53425-P (to M. J. v. R.), coordinated grants CTQ2015-64597-P-C02-01 and CTQ2015-64597-P-C02-02 (to J. J. B. and F. J. C., respectively), grant BFU2015-70052-R (to M. M.) and the Spanish Adenovirus Network (AdenoNet, BIO2015-68990-REDT), all from the Spanish Agencia Estatal de Investigación. Financial support to M. M. from the CIBER of Respiratory Diseases (CIBERES) from the Spanish Institute of Health Carlos III is also acknowledged. These grants are co-financed by the European Regional Development Fund of the European Union. A. K. S. and T. H. N. were recipients of pre-doctoral fellowships from La Caixa and CSIC-VAST, respectively. The expression vectors were designed and created in Hungary, and this was financed by the Hungarian Scientific Research Fund (OTKA K100163). M. K. thanks Enterprise Ireland for a Commercialisation Fund grant (CF/2015/0089), A. K. acknowledges the National University of Ireland for a Cancer Care West Hardiman PhD scholarship and L. J. acknowledges the EU FP7 programme in support of the GlycoHIT consortium (grant no. 260600). This work was supported by R01 AI104920 (to J. G. S.) from the National Institute for Allergy and Infectious Diseases (www.niaid.nih.gov). S. S. W. was also supported by the Helen Riaboff Whiteley Endowment to the University of Washington and by Public Health Service, National Research Service Awards T32 AI083203 from the National Institute for Allergy and Infectious Diseases and T32 GM007270 from the National Institute of General Medical Sciences.

Originally published in 1991, this volume discusses the urban working class, international migrants and the so-called lumpenproletariat. The book exhibits the fruitful interaction that has taken place between sociological theory, new views of the changing world economy and the empirical realities of working class experience and struggles. The dual theme of the book is the control which the state and employers seek to impose and maintain over labouring people, and the resistance put up by workers to these often new and unacceptable disciplines. With case studies - both historical and contemporary - drawn from North America, Britain and various parts of Africa, the author develops an interlocking theory of habituation and resistance. Against the background of profound changes in the global economy, Robin Cohen explores ways in which labouring people respond to the structural and managerial constrains on the development of their class consciousness and self-organisation. This will be of interest to urban and industrial sociologists, as well as those concerned with comparative social theory and the relationship between developing world and industrialised societies.

15 páginas, 6 figuras, 1 tabla. ; Basement membranes are extracellular structures of epithelia and endothelia that have collagen IV scaffolds of triple α-chain helical protomers that associate end-to-end, forming networks. The molecular mechanisms by which the noncollagenous C-terminal domains of α-chains direct the selection and assembly of the α1α2α1 and α3α4α5 hetero-oligomers found in vivo remain obscure. Autoantibodies against the noncollagenous domains of the α3α4α5 hexamer or mutations therein cause Goodpasture's or Alport's syndromes, respectively. To gain further insight into oligomer-assembly mechanisms as well as into Goodpasture's and Alport's syndromes, crystal structures of non-collagenous domains produced by recombinant methods were determined. The spontaneous formation of canonical homohexamers (dimers of trimers) of these domains of the α1, α3 and α5 chains was shown and the components of the Goodpasture's disease epitopes were viewed. Crystal structures of the α2 and α4 non-collagenous domains generated by recombinant methods were also determined. These domains spontaneously form homo-oligomers that deviate from the canonical architectures since they have a higher number of subunits (dimers of tetramers and of hexamers, respectively). Six flexible structural motifs largely explain the architectural variations. These findings provide insight into noncollagenous domain folding, while supporting the in vivo operation of extrinsic mechanisms for restricting the self-assembly of noncollagenous domains. Intriguingly, Alport's syndrome missense mutations concentrate within the core that nucleates the folding of the noncollagenous domain, suggesting that this syndrome, when owing to missense changes, is a folding disorder that is potentially amenable to pharmacochaperone therapy. ; The following funding is acknowledged: Spanish Government (Ministerio de Economia y Competitividad; grant Nos. BIO2013-42619 and BIO2016-78571-P to Alberto Marina; grant Nos. SAF 2009-10703, IPT-2011-1527-010000 and RTC2014-2415-1 to Juan Saus; grant No. BFU2016-78606-P to Patricia Casino; grant No. BFU2014-58229-P to Vicente Rubio; contract No. RYC-2014-16490 to Patricia Casino); Valencian Government Prometeo program (grant No. II/2014/029 to Alberto Marina, Vicente Rubio and Juan Saus); National Institutes of Health (grant NIH-DK18381 to Billy G. Hudson); European Community's Seventh Framework Programme FP7/2007-2013 (BioStruct-X; grant No. 283570); and a visiting researcher grant from the program 'Atraccio´ de Talent' of the University of Valencia to Billy G. Hudson. ; Peer reviewed