The Connection between Imported Intermediate Inputs and Exports: Evidence from Chinese Firms
In: NBER Working Paper No. w18260
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In: NBER Working Paper No. w18260
SSRN
In: Environmental science and pollution research: ESPR, Band 30, Heft 53, S. 114375-114390
ISSN: 1614-7499
In: PNAS nexus, Band 3, Heft 9
ISSN: 2752-6542
Abstract
The modular architecture of nonribosomal peptide synthetases (NRPSs) has inspired efforts to study their evolution and engineering. In this study, we analyze in detail a unique family of NRPSs from the defensive intracellular bacterial symbiont, Candidatus Endobryopsis kahalalidifaciens (Ca. E. kahalalidifaciens). We show that intensive and indiscriminate recombination events erase trivial sequence covariations induced by phylogenetic relatedness, revealing nonmodular functional constraints and clear recombination units. Moreover, we reveal unique substrate specificity determinants for multiple enzymatic domains, allowing us to accurately predict and experimentally discover the products of an orphan NRPS in Ca. E. kahalalidifaciens directly from environmental samples of its algal host. Finally, we expanded our analysis to 1,531 diverse NRPS pathways and revealed similar functional constraints to those observed in Ca. E. kahalalidifaciens' NRPSs. Our findings reveal the sequence bases of genetic exchange, functional constraints, and substrate specificity in Ca. E. kahalalidifaciens' NRPSs, and highlight them as a uniquely primed system for diversifying evolution.
In: Ecotoxicology and environmental safety: EES ; official journal of the International Society of Ecotoxicology and Environmental safety, Band 288, S. 117324
ISSN: 1090-2414
In: Environmental science and pollution research: ESPR, Band 30, Heft 14, S. 41609-41622
ISSN: 1614-7499
In: Ecotoxicology and environmental safety: EES ; official journal of the International Society of Ecotoxicology and Environmental safety, Band 107, S. 321-327
ISSN: 1090-2414
In: Environmental science and pollution research: ESPR, Band 31, Heft 2, S. 2759-2772
ISSN: 1614-7499
In: Ecotoxicology and environmental safety: EES ; official journal of the International Society of Ecotoxicology and Environmental safety, Band 181, S. 428-434
ISSN: 1090-2414
In: SCSI-D-24-08196
SSRN
In: Waste management: international journal of integrated waste management, science and technology, Band 186, S. 46-54
ISSN: 1879-2456
In: WM-23-3466
SSRN
In: STOTEN-D-21-31165
SSRN
In: Ecotoxicology and environmental safety: EES ; official journal of the International Society of Ecotoxicology and Environmental safety, Band 197, S. 110643
ISSN: 1090-2414
In: Ecotoxicology and environmental safety: EES ; official journal of the International Society of Ecotoxicology and Environmental safety, Band 172, S. 40-44
ISSN: 1090-2414
In: Ecotoxicology and environmental safety: EES ; official journal of the International Society of Ecotoxicology and Environmental safety, Band 225, S. 112796
ISSN: 1090-2414