This study is part of the program of the interdisciplinary research group Profetas (protein foods, environment, technology and society). Profetas consists of technological, environmental and socio-economic research projects on protein food systems which result in the development of scenarios and strategies for guiding a shift towards a more plant protein based diet. The different research projects focus on the goal of identifying viable options for a more sustainable food system. Profetas arose from a concern with the increasing impact of food production and consumption on the environment and a desire to restrain human pressure on the environment.
Commercial Protein Supplements that are circulating freely nowadays are consumed not only by athletes but the wider community, especially sports activists who rely upon it to improve performance or merely for aesthetic interests. This study aims to analyze the nutritional content of the five supplements circulating freely in Indonesia to then compare it with the claims on the packaging label. We analyzed five samples of popular supplements in Indonesia obtained online and then tested in three copies for each sample. The actual total protein in the supplement sample is measured using the Kjeldahl method where the appropriate nitrogen material is converted to percentage of protein by multiplying a factor of 6.38 in duplicate. Then the research data processed and analyzed using Microsoft Excel 2016 computer programs then by Statistical Program for Social Sciences (SPSS), consecutively. Relationships between different tests were evaluated by calculating the relevant correlation coefficients (Pearson linear correlation) (p <0.05). Analysis of variance (ANOVA) was used to compare the differences between products with different manufacturing processes (p <0.05). Based on the analysis results, only two of the five samples studied has a minimum of 80% compatibility with the claim on the packaging label. Several main factors including inaccurate analysis, preparation processing on products that triggers protein denaturation, inadequate storage, and regulations that have just been set so that producers are still free to write Nutrition Value Information with a fairly low level of accuracy. This research is expected to be able to help the evaluation for producers and the government (BPOM) to be able to provide correct information to consumers so that maximum physiological benefits can be obtained. ; Commercial Protein Supplements that are circulating freely nowadays are consumed not only by athletes but the wider community, especially sports activists who rely upon it to improve performance or merely for aesthetic interests. This study aims to analyze the nutritional content of the five supplements circulating freely in Indonesia to then compare it with the claims on the packaging label. We analyzed five samples of popular supplements in Indonesia obtained online and then tested in three copies for each sample. The actual total protein in the supplement sample is measured using the Kjeldahl method where the appropriate nitrogen material is converted to percentage of protein by multiplying a factor of 6.38 in duplicate. Then the research data processed and analyzed using Microsoft Excel 2016 computer programs then by Statistical Program for Social Sciences (SPSS), consecutively. Relationships between different tests were evaluated by calculating the relevant correlation coefficients (Pearson linear correlation) (p <0.05). Analysis of variance (ANOVA) was used to compare the differences between products with different manufacturing processes (p <0.05). Based on the analysis results, only two of the five samples studied has a minimum of 80% compatibility with the claim on the packaging label. Several main factors including inaccurate analysis, preparation processing on products that triggers protein denaturation, inadequate storage, and regulations that have just been set so that producers are still free to write Nutrition Value Information with a fairly low level of accuracy. This research is expected to be able to help the evaluation for producers and the government (BPOM) to be able to provide correct information to consumers so that maximum physiological benefits can be obtained.
[EN] One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organically-modified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that greater GPTMS surface concentrations induced greater secretion of TNF-alpha and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP) and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentration-dependent. Our results show that a greater adsorption of complement proteins can condition macrophage polarization. ; This work was supported by MINECO [MAT2017-86043-R]; Universitat Jaume I [Predoc/2014/25, UJI-B2017-37]; Basque Government [IT611-13, Predoc/2016/1/0141]; University of the Basque Country [UFI11/56]; CIC bioGUNE is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), ProteoRed-ISCIII [PRB3 IPT17/0019]; CIBERehd Network and Severo Ochoa Grant [SEV-2016-0644]. Authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver, Jose Ortega (UJI), René van Rheden, Vicent Cuijpers (Radboudumc) and Iraide Escobes (CIC bioGUNE) for their valuable technical assistance. ; Araújo-Gomes, N.; Romero-Gavilán, F.; Zhang, Y.; ...
125 133 181 ; S ; [EN] One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organically-modified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that greater GPTMS surface concentrations induced greater secretion of TNF-alpha and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP) and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentration-dependent. Our results show that a greater adsorption of complement proteins can condition macrophage polarization. This work was supported by MINECO [MAT2017-86043-R]; Universitat Jaume I [Predoc/2014/25, UJI-B2017-37]; Basque Government [IT611-13, Predoc/2016/1/0141]; University of the Basque Country [UFI11/56]; CIC bioGUNE is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), ProteoRed-ISCIII [PRB3 IPT17/0019]; CIBERehd Network and Severo Ochoa Grant [SEV-2016-0644]. Authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver, Jose Ortega (UJI), René van Rheden, Vicent Cuijpers (Radboudumc) and Iraide Escobes (CIC bioGUNE) for their valuable technical assistance. Araújo-Gomes, N.; Romero-Gavilán, F.; Zhang, ...
Computational prediction of protein–protein complex structure by docking can provide structural and mechanistic insights for protein interactions of biomedical interest. However, current methods struggle with difficult cases, such as those involving flexible proteins, low-affinity complexes or transient interactions. A major challenge is how to efficiently sample the structural and energetic landscape of the association at different resolution levels, given that each scoring function is often highly coupled to a specific type of search method. Thus, new methodologies capable of accommodating multi-scale conformational flexibility and scoring are strongly needed. We describe here a new multi-scale protein–protein docking methodology, LightDock, capable of accommodating conformational flexibility and a variety of scoring functions at different resolution levels. Implicit use of normal modes during the search and atomic/coarse-grained combined scoring functions yielded improved predictive results with respect to state-of-the-art rigid-body docking, especially in flexible cases. ; B.J-G was supported by a FPI fellowship from the Spanish Ministry of Economy and Competitiveness. This work was supported by I+D+I Research Project grants BIO2013-48213-R and BIO2016-79930-R from the Spanish Ministry of Economy and Competitiveness. This work is partially supported by the European Union H2020 program through HiPEAC (GA 687698), by the Spanish Government through Programa Severo Ochoa (SEV-2015-0493), by the Spanish Ministry of Science and Technology (TIN2015-65316-P) and the Departament d'Innovació, Universitats i Empresa de la Generalitat de Catalunya, under project MPEXPAR: Models de Programaciói Entorns d'Execució Paral·lels (2014-SGR-1051). ; Peer Reviewed ; Postprint (author's final draft)
