Morphogenesis in vitro in calluses of lavender Lavandula angustifolia Mill.: histological aspects

In: Izvestiâ Akademii nauk. Rossijskaâ akademiâ nauk. Seriâ biologičeskaâ, Heft 3, S. 297-306

Abstract

Histological events occurring in the calluses of Lavandula angustifolia Mill. at the initial stages of in vitro culture (1 passage) were described for the first time. It was found that the non-morphogenic callus is mainly represented by parenchymal tissue with few morphogenetic foci, mostly degenerated. In morphogenic calluses the morphogenesis pathways such as de novo organogenesis and indirect in vitro somatic embryogenesis have been identified and multiple developing morphogenetic foci have been noted also. The question of the realization of the pluri- and totipotency properties of callus cells in vitro is discussed. The histological data can be used in choosing the direction of application of regenerants obtained from calluses of this valuable essential oil and medicinal plant in various cell technologies.