145 Sampling Evaluation of Bacteria antibiotic-resistance from air and oral samples in surgical and medical intensive care units

In: Annals of work exposures and health: addressing the cause and control of work-related illness and injury, Band 68, Heft Supplement_1, S. 1-1

Abstract

Abstract
The antibiotic-resistance of bioaerosols and bacteria from patients' mouth were evaluated in surgical and medical intensive care units (ICUs). A six-stage viable Andersen cascade impactor was used to collect bioaerosols. The sterilized cotton swabs were used to collect oral samples from four randomly selected patient's mouths. Antibiotic susceptibility tests (AST) were performed using a BD Phoenix-100 automated interpretation system. The minimum inhibitory concentrations of the antimicrobial agents for these bacteria isolates were determined using the BD Phoenix-100 instrument.
A total of 199 bacterial isolates were tested for the AST, of which 92.8% were Gram-positive bacteria. The dominated bacteria was Staphylococcus spp. (71.3%), and the others were Micrococcus spp. (15%), Burkholderia spp. (3 %) and Bacillus spp. (2%). According to the AST, 75.9% of sampled airborne bacteria in medical and surgical ICUs were recognized as antibiotic-resistant bacteria.
The results of oral samples showed that a total of 3 over 6 bacteria species revealed multidrug resistant to antibiotics. Pseudomonas aeruginosa was found to resist 9 type of antibiotics, such as AM, SAM (Penicillin), CZ, CTX, CRO (cephalosporins), ET (carbapenem), MINO (Tetracycline TCs), TGCs (glycylcycline), and TMP-SMX (co-trimoxazole). Burkholderia cepacia and Ralstonia pickettii were found to resist 5 antibiotics: AM, SAM (Penicillin), CZ (cephalosporins), CL (Polypeptide), and ETPs (carbapenems).
The presence of antibiotic resistance in ICUs poses a potential risk to the health of medical staff and patients. Therefore, it is recommended that the ventilation design of ICUs should be evaluated to ensure the effectiveness of nosocomial control.